Anti-ERp57 Antibody (E-7) recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ERp57 Antibody (E-7): sc-133168

Anti-ERp57 Antibody (E-7) is rated 5.0 out of 5 by 3.
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Datasheets

    • ERp57 Antibody (E-7) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 108-207 mapping to an internal region of ERp57 of human origin
    • recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ERp57 Antibody (E-7). This work is in progress.
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Can ERp57 (E-7): sc-133168 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, ERp57 (E-7): sc-133168 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Positive result in WB in non Positive result in WB in non-transfected 293T, human ERp57 transfected 293T and Daudi whole cell lysates. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by from Strong band of interest seen in WB in Caki Strong band of interest seen in WB in Caki-1 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-01-12
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