ERp46 Antibody (F-3)recommended for detection of ERp46 of human origin by WB, IP, IF, IHC(P) and ELISA

ERp46 Antibody (F-3): sc-271465

ERp46 Antibody (F-3) is rated 5.0 out of 5 by 3.
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Datasheets

    • ERp46 Antibody (F-3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 137-216 mapping within an internal region of ERp46 of human origin
    • recommended for detection of ERp46 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can ERp46 (F-3): sc-271465 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, ERp46 (F-3): sc-271465 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Strong band seen in WB in HeLa whole cell lysate Strong band seen in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-06-03
Rated 5 out of 5 by from Strong band detected in WB in 293T whole Strong band detected in WB in 293T whole cell lysate. -SCBT QC
Date published: 2015-02-28
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2015-02-09
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