Anti-ERK 1 Antibody (E-6) recommended for detection of ERK 1 p44 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ERK 1 Antibody (E-6): sc-271270

Anti-ERK 1 Antibody (E-6) is rated 4.5 out of 5 by 10.
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Datasheets

    • ERK 1 Antibody (E-6) is a mouse monoclonal IgG1 κ, cited in 30 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 291-335 within subdomain XI of ERK 1 of rat origin
    • Anti-ERK 1 Antibody (E-6) is recommended for detection of ERK 1 p44 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-ERK 1 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERK 1 (E-6): sc-271270.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ERK 1 Antibody (E-6) for WB and IHC(P) applications. These reagents are now offered in bundles with ERK 1 Antibody (E-6) (see ordering information below).
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What application is the blocking peptide sc-271270 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good in WB The quality of this antibody is good in western blotting. I get clear bands by using 1:1000.
Date published: 2021-07-21
Rated 5 out of 5 by from Excellent I had a great signal and very specific. No unspecific binding was observed
Date published: 2021-03-22
Rated 4 out of 5 by from Worked well. Worked well for Western application against mouse samples.
Date published: 2018-03-23
Rated 5 out of 5 by from ERK 1 Western Blot Primary cerebellar neurons conjugated with ERK 1 (1:1000). Very clear signal with little background.
Date published: 2018-03-09
Rated 2 out of 5 by from Okay for western blot Used this antibody 1:1000 on a western blot with 100 ug of protein from tissue. Band was relatively obvious, but very weak. Compared this to identical western blot run side by side probed with Erk1 sc-271269 which was much better.
Date published: 2018-02-21
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of ERK 1 expression in HeLa and MCF7 whole cell lysates.
Date published: 2017-02-01
Rated 4 out of 5 by from This has worked well in WB This has worked well in WB.
Date published: 2015-11-17
Rated 5 out of 5 by from IF: Very good nuclear and cytoplasmic staining IF: Very good nuclear and cytoplasmic staining in HepG2 cells. -SCBT QC
Date published: 2015-03-27
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