Anti-ERK 1/2 Antibody (C-9) recommended for detection of ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ERK 1/2 Antibody (C-9): sc-514302

Anti-ERK 1/2 Antibody (C-9) is rated 4.9 out of 5 by 66.
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Datasheets

    • Anti-ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG2a κ ERK 1/2 antibody, cited in 348 publications, provided at 200 µg/ml
    • raised against amino acids 101-172 mapping near the N-terminus of ERK 2 of human origin
    • Anti-ERK 1/2 Antibody (C-9) is recommended for detection of ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ERK 1/2 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERK 1/2 (C-9): sc-514302.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ERK 1/2 Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ERK 1/2 Antibody (C-9) (see ordering information below).
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I used this antibody to probe with WB on TF-1 cell line and found one band about the right size (~40kDa) but got an extra band about 25 kDa. My secondary antibody is also from yours (sc-2005). Do you have any ideas about the extra band?

Asked by: SCUser
Thank you for your question. We recommend running a secondary antibody control (omit primary) blot to see if that non-specific band is from your secondary antibody alone. It is possible you are detecting light chain IgG at that molecular weight.
Answered by: Technical Support
Date published: 2019-02-05

Does the antibody detects total ERK and the Phosporylated form?

Asked by: Rodrigo lizarraga
Thank you for your question. Yes, this can detect the phosphorylated and non phosphorylated form of ERK1/2.
Answered by: Technical Support
Date published: 2019-01-06

Is this antibody cross-reactive with Erk 3?

Asked by: AbPolly
Thank you for your inquiry. Our ERK 1/2 antibody sc-514302 is not known to cross-react with ERK3. For further details or other questions about reactivity, please contact our Technical Services Department.
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 5 out of 5 by from Great experience Very high quality. I am very content with the product. Thank you.
Date published: 2021-09-16
Rated 5 out of 5 by from ERK This primary antibody was able to detect total ERK (p42 and p44) giving well defined bands.
Date published: 2021-05-17
Rated 5 out of 5 by from WB We us this antibody with 1:600, the result is very good.
Date published: 2020-08-22
Rated 5 out of 5 by from Excellent antibody for western blotting ERK1/2(C-9):sc-514302 is good quality for western blotting (dilute factor: 1:1000)
Date published: 2020-05-25
Rated 4 out of 5 by from ノンスぺがほとんどなく、ターゲットを明確に検出することができた サンプルを受け取り、HeLa, LU99細胞で刺激による変化を解析した。いずれの細胞でもはっきりとしたバンドとして検出することができた。
Date published: 2019-11-25
Rated 5 out of 5 by from great antibody santa cruz antibodies are easy to work with and are not expensive
Date published: 2019-11-05
Rated 5 out of 5 by from 发货很快,条带很清晰 我一直购买santa的抗体,涉及IP/WB/Chip值得信赖,希望可以继续保持这样的品质,一直这样下去
Date published: 2019-10-31
Rated 5 out of 5 by from Ottimo anticorpo Ho provato questo anticorpo su un estratto totale di cellule HeLa usando una diluizione 1:100 e ha riconosciuto molto bene la proteina di interesse!
Date published: 2019-09-02
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