Anti-ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG2a κ ERK 1/2 antibody, cited in 385 publications, provided at 200 µg/ml
raised against amino acids 101-172 mapping near the N-terminus of ERK 2 of human origin
Anti-ERK 1/2 Antibody (C-9) is recommended for detection of ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-ERK 1/2 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERK 1/2 (C-9): sc-514302.
m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ERK 1/2 Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ERK 1/2 Antibody (C-9) (see ordering information below).
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ERK 1/2 Antibody (C-9) is a high quality monoclonal ERK 1/2 antibody (also designated p44/42 MAPK antibody) suitable for the detection of the ERK 1/2 protein of mouse, rat and human origin. ERK 1/2 Antibody (C-9) is available as both the non-conjugated anti-ERK 1/2 antibody form, as well as multiple conjugated forms of anti-ERK 1/2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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