eRF1 Antibody (F-1)recommended for detection of eRF1 of mouse, rat, human and avian origin by WB, IP, IF and ELISA

eRF1 Antibody (F-1): sc-390427

eRF1 Antibody (F-1) is rated 5.0 out of 5 by 1.
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Datasheets

    • eRF1 Antibody (F-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of eRF1 of human origin
    • recommended for detection of eRF1 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using eRF1 (F-1): sc-390427?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced positive Western blot data of eRF1 Produced positive Western blot data of eRF1 expression in SK-OV-3 and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-03-01
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