Anti-ErbB4/HER4 Antibody (HFR1) recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ErbB4/HER4 Antibody (HFR1): sc-53280

Anti-ErbB4/HER4 Antibody (HFR1) is rated 5.0 out of 5 by 3.
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Datasheets

    • ErbB4/HER4 Antibody (HFR1) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1249-1264 of ErbB-4 of human origin
    • recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See ErbB4/HER4 (C-7): sc-8050 for ErbB4/HER4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ErbB4/HER4 Antibody (HFR1) for WB and IHC(P) applications. These reagents are now offered in bundles with ErbB4/HER4 Antibody (HFR1) (see ordering information below).
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I am using ErbB-4 (HFR1): sc-53280 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Robust Robust, clean band observed in western blot using ErbB-4 transfected NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from Published immunocytochemical data using HFR1 Published immunocytochemical data using HFR1 on human MPNST cells. -SCBT Publication Review
Date published: 2015-05-11
Rated 5 out of 5 by from Worked well for WB using mpkCCDc14 cell lysates Worked well for WB using mpkCCDc14 cell lysates. -SCBT Publication Review
Date published: 2014-08-19
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