Anti-ErbB4/HER4 Antibody (C-7) recommended for detection of ErbB-4 of mouse, rat, human and origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-ErbB4/HER4 Antibody (C-7): sc-8050

Anti-ErbB4/HER4 Antibody (C-7) is rated 4.3 out of 5 by 13.
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Datasheets

    • Anti-ErbB4/HER4 Antibody (C-7) is a mouse monoclonal IgG2a κ ErbB4/HER4 antibody, cited in 47 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1291-1308 at the C-terminus of ErbB-4 of human origin
    • ErbB4/HER4 Antibody (C-7) is recommended for detection of ErbB-4 of mouse, rat, human and origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-ErbB4/HER4 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ErbB4/HER4 (C-7): sc-8050.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ErbB4/HER4 Antibody (C-7) for WB and IHC(P) applications. These reagents are now offered in bundles with ErbB4/HER4 Antibody (C-7) (see ordering information below).
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What application is the blocking peptide sc-8050 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 5
Date published: 2017-01-22
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Rated 1 out of 5 by from Did not work for immunofluorescence (pig) Immunohistochemistry: the antibody was tested on mammary gland and kidney from 6 months old gilts. Antigen retrieval was performed in citrate buffer, 30'. Primary antibody: 1:50 dilution, 1h, RT. Secondary antibody: Alexa Fluor 488 (1:500), 1h, RT. Results: negative; nonspecific signal was observed both in epithelial and connective fractions.
Date published: 2021-02-01
Rated 5 out of 5 by from Works very well for flow cytommetry We purchased this item for our flow cytommetry experiments. We are very pleased with the results.
Date published: 2018-04-23
Rated 5 out of 5 by from The baseband is perfect ErbB-4 Antibody is the best I have ever used.We got obtained specific band is very clear and signal is powerful. The effect is amazing.
Date published: 2017-10-17
Rated 5 out of 5 by from Western blotting in mouse Western Blot, 1:500 dilution, it works well, and the detection efficiency of this antibody is very high, I love this antibody.
Date published: 2017-05-22
Rated 5 out of 5 by from Very popular choice! The (C-7) clone for ErbB-4 is very well characterized with over 30 known citations in the literature using the antibody for a variety of research applications.
Date published: 2016-12-22
Rated 5 out of 5 by from Detection efficiency is very good Our laboratory likes to use this antibody, Western blot, 1:200 dilution, it works well, and this antibody detection efficiency is very high.
Date published: 2016-12-21
Rated 4 out of 5 by from We use this monoclonal a lot in WB and IF We use this monoclonal a lot in WB and IF and we have published with it. Has not failed us yet!
Date published: 2015-09-11
Rated 1 out of 5 by from Used in Western Blot application; No signal Used in Western Blot application; No signal observed on the Blot.
Date published: 2015-05-20
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