Anti-ErbB4/HER4 Antibody (3F168) recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ErbB4/HER4 Antibody (3F168): sc-71070

Anti-ErbB4/HER4 Antibody (3F168) is rated 4.5 out of 5 by 2.
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Datasheets

    • ErbB4/HER4 Antibody (3F168) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1249-1264 of ErbB-4 of human origin
    • recommended for detection of ErbB-4 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See ErbB4/HER4 (C-7): sc-8050 for ErbB4/HER4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ErbB4/HER4 Antibody (3F168). This work is in progress.
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Can ErbB-4 (3F168): sc-71070 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, ErbB-4 (3F168): sc-71070 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works well for WB with T47 Works well for WB with T47-14 whole cell lysate. -SCBT QC
Date published: 2015-06-09
Rated 4 out of 5 by from Published IHC and WB data using SC Published IHC and WB data using SC-71070 with mouse brain sections and extracts.
Date published: 2013-12-14
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