Anti-ErbB3/HER3 Antibody (RTJ.2) recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-ErbB3/HER3 Antibody (RTJ.2): sc-415

Anti-ErbB3/HER3 Antibody (RTJ.2) is rated 5.0 out of 5 by 6.
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Datasheets

    • ErbB3/HER3 Antibody (RTJ.2) is a mouse monoclonal IgG1 κ, cited in 57 publications, provided at 200 µg/ml
    • epitope mapping within the cytoplasmic domain of ErbB-3 of human origin
    • Anti-ErbB3/HER3 Antibody (RTJ.2) is recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-ErbB3/HER3 Antibody (RTJ.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ErbB3/HER3 (RTJ.2): sc-415.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ErbB3/HER3 Antibody (RTJ.2) for WB and IHC(P) applications. These reagents are now offered in bundles with ErbB3/HER3 Antibody (RTJ.2) (see ordering information below).
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Can ErbB-3 (RTJ.2): sc-415 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ErbB-3 (RTJ.2): sc-415 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Published results of immunoprecipitation Published results of immunoprecipitation using human lung cancer cell lines. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Worked well for IHC pn CJ7 embryonic stem cells Worked well for IHC pn CJ7 embryonic stem cells. -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from Exceptional immunofluorescence membrane staining Exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Published immunohistochemical analysis of Published immunohistochemical analysis of paraffin embedded non-neoplastic urothelium and chronic pyelonephritis chronic ureteritis slices, kidney laceration slices and uretero-pelvic junction stenosis sections. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Robust signal in Western blot analysis of ErbB Robust signal in Western blot analysis of ErbB-3 expression in SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-01-03
Rated 5 out of 5 by from Exceptional intracellular FCM data of fixed Exceptional intracellular FCM data of fixed and permeabilized HEL 92.1.7 cells. -SCBT QC
Date published: 2013-12-12
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