Anti-ErbB3/HER3 Antibody (G-4) recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

Anti-ErbB3/HER3 Antibody (G-4): sc-7390

Anti-ErbB3/HER3 Antibody (G-4) is rated 3.9 out of 5 by 11.
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Datasheets

    • Anti-ErbB3/HER3 Antibody (G-4) is a mouse monoclonal IgG2b κ ErbB3/HER3 antibody, cited in 27 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1315-1342 at the C-terminus of ErbB-3 of human origin
    • Anti-ErbB3/HER3 Antibody (G-4) is recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including and bovine and porcine
    • Anti-ErbB3/HER3 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ErbB3/HER3 (G-4): sc-7390.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ErbB3/HER3 Antibody (G-4) for WB applications. These reagents are now offered in bundles with ErbB3/HER3 Antibody (G-4) (see ordering information below).
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Is this erbb3 antibody should be applied as intercellular staining ? or as surface staining

Asked by: changl
Thank you for your question. ErbB-3 Antibody (G-4): sc-7390 is specific against amino acids 1315-1342. The C-terminal amino acid range between 665 – 1342 is cytoplasmic, so the antibody would recognize only the cytoplasmic part of its target protein.
Answered by: Tech Support Europe
Date published: 2020-09-22

How should the blocking peptide sc-7390 P be used?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended to be used as a negative control by preadsorbing the ErbB-3 (G-4): sc-7390 monoclonal antibody with it prior to application. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 1 out of 5 by from Did not work for immunofluorescence (pig) Immunohistochemistry: the antibody was tested on mammary parenchyma and uterus from 6 months old female pigs. Antigen retrieval was performed in citrate buffer, 30'. Primary antibody: 1:50 dilution, 1h, RT. Secondary antibody: Alexa Fluor 488 (1:500), 1h, RT. Results: negative; nonspecific signal was observed both in epithelial and connective fractions. Immunocytochemistry: the antibody was tested on porcine mammary epithelial cells isolated from 6 months old gilts. Cells were fixed with acetone-methanol (1:1) and stained as above (see immunohistochemistry). Primary antibody was diluted 1:100. Result: negative.
Date published: 2021-02-01
Rated 1 out of 5 by from Didnot work for WB did not work for western - cells were human cells in culture
Date published: 2017-11-21
Rated 1 out of 5 by from Did not work for IP I requested a sample of this antibody to IP HER3 from human colorectal cancer cells with 1ug antibody per 200ug of proteins from cell lysate. HER3 in these cells were able to be IP pulled down or detected by Western blotting with a HER3 antibody carried by Cell Signaling.
Date published: 2017-07-19
Rated 5 out of 5 by from Superb product !! Very clean western blot data; strong signal, low background; also strong IF staining data
Date published: 2017-02-04
Rated 5 out of 5 by from Perfect antibody! This is a great choice if you are looking for a solid and versatile antibody for detecting ErbB-3. It's strong not only for WB, but also for IF and FCM applications.
Date published: 2016-12-20
Rated 5 out of 5 by from Published data using pfa fixed spinal cord Published data using pfa fixed spinal cord sections from rat - immunohistochemical analysis. -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from Worked well for IP using A Worked well for IP using A-431 and MDA-MB-468 cell lysates. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from No comment No comment.
Date published: 2015-03-27
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