Anti-ErbB3/HER3 Antibody (2Q320) recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-ErbB3/HER3 Antibody (2Q320): sc-71067

Anti-ErbB3/HER3 Antibody (2Q320) is rated 3.0 out of 5 by 1.
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Datasheets

    • ErbB3/HER3 Antibody (2Q320) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • epitope mapping within the cytoplasmic domain of ErbB-3 of human origin
    • recommended for detection of ErbB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • See ErbB3/HER3 (G-4): sc-7390 for ErbB3/HER3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ErbB3/HER3 Antibody (2Q320) for WB and IHC(P) applications. These reagents are now offered in bundles with ErbB3/HER3 Antibody (2Q320) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using ErbB-3 (2Q320): sc-71067?

Asked by: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Positive results seen in human ErbB Positive results seen in human ErbB-3 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-02-23
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