ErbB-3 Antibody (11A4) recommended for detection of ErbB-3 of human origin by WB and IP

ErbB-3 Antibody (11A4): sc-81454

ErbB-3 Antibody (11A4) is rated 4.3 out of 5 by 3.
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Datasheets

    • ErbB-3 Antibody (11A4) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against the C-terminus of ErbB-3 of human origin
    • recommended for detection of ErbB-3 of human origin by WB and IP
    • See ErbB-3 (G-4): sc-7390 for ErbB-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

I am using ErbB-3 (11A4): sc-81454 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Strong signal in Western blot analysis of ErbB Strong signal in Western blot analysis of ErbB-3 expression in human ErbB-3 transfected NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Clear bands observed on immunoblot; used Clear bands observed on immunoblot; used human ErbB-3 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Robust Robust, singular band observed in Western blot analysis of ErbB-3 expression in T-47D whole cell lysate. -SCBT QC
Date published: 2014-02-20
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