Anti-EpoR Antibody (D-5) recommended for detection of EpoR of human origin by WB, IP, IF and ELISA

Anti-EpoR Antibody (D-5): sc-365662

Anti-EpoR Antibody (D-5) is rated 4.6 out of 5 by 7.
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Datasheets

    • Anti-EpoR Antibody (D-5) is a mouse monoclonal IgG3 κ EpoR antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 491-513 within a C-terminal cytoplasmic domain of EpoR of human origin
    • recommended for detection of EpoR of human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EpoR (D-5): sc-365662.
    • m-IgG3 BP-HRP is the preferred secondary detection reagent for EpoR Antibody (D-5) for WB applications. This reagent is now offered in a bundle with EpoR Antibody (D-5) (see ordering information below).
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I used to use M-20 and C-20 to detect total EPOR in my IP samples, however, they are all discontinued, i tried this D-5 many times, but it doesn´t work at all. Do you have other recommendations? I use human cell line ASE2.

Asked by: ck5458
Thank you for your question. We are sorry to hear that this antibody is not performing well in your experiments. We currently do not offer any other antibodies specific against this epitope. If you contact our technical service, we may be able to help you optimize your protocol and make the antibody work. You can also find our recommended protocol for IP by following this link: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support Europe
Date published: 2019-02-19

All of the other antibodies for rat EPO-R of your company are now discontinued. Does this antibody sc-365662 cross-react with rat EPO-R in western blot ?

Asked by: Asaine
Thank you for your question. We have not yet tested this antibody, EpoR (D-5): sc-365662, for reactivity with EpoR of mouse or rat origin. Therefore, it is not a recommended species at this time. Unfortunately, we do not offer any alternative antibodies for rat EpoR at this time.
Answered by: Technical Support
Date published: 2017-10-04

Is this antibody good for flow cytometry?

Asked by: Yenling
Thank you for your question. Unfortunately this antibody has not been tested for Flow Cytometry. However, we would be glad to send you a sample of it to try, because it is definitely possible that it works great for Flow cytometry, it just has not been tested. If you would like to try a sample, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-20

What application is the blocking peptide sc-365662 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Free sample worked okay! Thanks Santacruz! The free sample of your antibody worked okay for IHC (1:100)
Date published: 2018-02-22
Rated 4 out of 5 by from Nice worked on tissue homogenate I used it on WB of kidney tissue homogenate. It worked well with few unspecific binding. However, when I apply it to tubular cell line lysis in WB, I did not see specific band.
Date published: 2017-07-14
Rated 5 out of 5 by from play good WB We used this antibody 1:1000 for WB and got a specific band between 55kd and 70kd.
Date published: 2017-04-29
Rated 5 out of 5 by from IHC I used this antibody in IHC (dilution 1:100) and got good staining in human stomach tissue.
Date published: 2017-02-25
Rated 5 out of 5 by from A clean band can be seen. Antibody use many times, bought two antibodies but different batches this time, the quality is stable.
Date published: 2017-02-13
Rated 5 out of 5 by from Good for WB The EpoR Antibody (D-5): sc-365662 provides good data for Western blotting applications, showing strong band with minimal background.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced nice Western blot data of EpoR expression Produced nice Western blot data of EpoR expression in GM-CSF treated K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-05-21
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