ephrin-B2 Antibody (F-2)recommended for detection of ephrin-B2 of mouse, rat and human origin by WB, IP, IF and ELISA

ephrin-B2 Antibody (F-2): sc-398735

ephrin-B2 Antibody (F-2) is rated 3.4 out of 5 by 5.
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Datasheets

    • ephrin-B2 Antibody (F-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 168-235 of ephrin-B2 of human origin
    • ephrin-B2 Antibody (F-2) is recommended for detection of ephrin-B2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • ephrin-B2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ephrin-B2 (F-2): sc-398735.
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Can ephrin-B2 (F-2): sc-398735 monoclonal antibody be used to detect the extracellular fragments of EphrinB2

Asked by: niniling
Thank you for your question. The epitope for ephrin-B2 (F-2), sc-398735, maps within amino acids 168-235, however the exact epitope has not been mapped. The extracellular region for this protein is amino acids 28-229, so chances are very good the epitope is within the extracellular region.
Answered by: Tech Service
Date published: 2019-06-04

I have got sc-398735. However, there is no result in IF. Is the second antibody (such as sc-398735 AF488 and sc-398735 AF647 ) must be used?

Asked by: tun0423
Thank you for your question. It is not necessary to use the conjugated version of this mouse monoclonal primary antibody, ephrin-B2 (F-2): sc-398735, for immunofluorescence (IF). You can use a conventional anti-mouse IgG secondary antibody for detection, or one of our mouse IgG kappa light chain binding proteins (e.g., m-IgGκ BP-CFL 488: sc-516176). A link to our recommended protocol for IF with this antibody is provided below. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you still have no result with the antibody, please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com> or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-04-28

I have 2 questions: 1. Is it antibody can be used for IHC(P)? we have "Dako EnVision Dual Link System-HRP" as the second antibody. 2. If it can be used in IHC(P), which isotype control antibody is suggested? Thank u so much!

Asked by: Tun W
Thank you for your question. We have not yet tested this mouse monoclonal, ephrin-B2 Antibody (F-2): sc-398735, on formalin-fixed paraffin-embedded tissue sections, or for IHC(P) so we do not know if the antibody is suitable for this application. If you want to test the antibody for IHC(P), the antibody has an isotype of mouse monoclonal IgG2a so the corresponding isotype control is our, normal mouse IgG2a: sc-3878. https://www.scbt.com/scbt/product/normal-mouse-igg2a
Answered by: Technical Support
Date published: 2017-04-04

Can ephrin-B2 (F-2): sc-398735 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ephrin-B2 (F-2): sc-398735 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-09-05
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Rated 1 out of 5 by from not good for immune-staining in brain section I used this trial recently and do IF in mice brain section but not good with the solution 1:100 and 1:50. Very little signals were seen. I use this as a marker for brain arterial vessel, it should be a lot under the scope.
Date published: 2018-04-22
Rated 5 out of 5 by from 条带很清晰,也很特异,很好用。 Western blot做出来的结果条带很特异,也很清晰,基本上没有杂带,几次试验下来结果都很稳定。
Date published: 2017-10-13
Rated 1 out of 5 by from This antibody doesn't work for mouse brain on WB This antibody doesn't show band around ~37kd, only has two strong bands at size ~23kd and ~50kd for mouse brain cortex samples on WB.
Date published: 2017-03-09
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of ephrin-B2 expression in PANC-1, NIH/3T3 and NRK whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from Very good Western blot data of ephrin Very good Western blot data of ephrin-B2 expression in HT-29, HUV-EC-C and RT-4 whole cell lysates. -SCBT QC
Date published: 2014-01-17
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