ephrin-A3 Antibody (12EXZ)recommended for detection of ephrin-A3 extracellular domain of human origin by WB and IP

ephrin-A3 Antibody (12EXZ): sc-73954

ephrin-A3 Antibody (12EXZ) is rated 3.0 out of 5 by 3.
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Datasheets

    • ephrin-A3 Antibody (12EXZ) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against the extracellular domain of ephrin-A3 of human origin
    • recommended for detection of ephrin-A3 extracellular domain of human origin by WB and IP
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I am using ephrin-A3 (12EXZ): sc-73954 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Published using this antibody for ChIP Published using this antibody for ChIP. -SCBT Publication Review
Date published: 2015-01-23
Rated 3 out of 5 by from WB: worked well with HeLa WB: worked well with HeLa, HUVEC, MCF-7, and MDA-MB231 cell lysates.
Date published: 2015-01-02
Rated 3 out of 5 by from Robust bands in transfected lysates Robust bands in transfected lysates. -SCBT QC
Date published: 2013-06-10
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