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Eosin Y (CAS 15086-94-9)

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Application:
Eosin Y is a brominated fluoroscein dye derivative
CAS Number:
15086-94-9
Molecular Weight:
647.89
Molecular Formula:
C20H8Br4O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Eosin Y is a fluorescent compound widely used in the field of microscopy and histology as a stain for highlighting cytoplasmic material, collagen, and muscle fibers in tissue sections. In biological research, it is paired with hematoxylin to perform H&E (hematoxylin and eosin) staining, which is a standard technique for revealing cellular and tissue structures. Eosin Y′s affinity for eosinophilic structures, which are components that tend to have a net positive charge, makes it a vital tool for distinguishing different cell types and morphological features. In addition to its application in staining, Eosin Y is also used in photodynamic research due to its ability to generate singlet oxygen when exposed to light of a specific wavelength. The compound′s fluorescent properties are harnessed in various assays to investigate cell viability, apoptosis, and other cellular functions.


Eosin Y (CAS 15086-94-9) References

  1. An eosin Y-based 'turn-on' fluorescent sensor for detection of perfluorooctane sulfonate.  |  Liang, J., et al. 2015. Spectrochim Acta A Mol Biomol Spectrosc. 150: 772-7. PMID: 26103430
  2. Eosin Y as a Direct Hydrogen-Atom Transfer Photocatalyst for the Functionalization of C-H Bonds.  |  Fan, XZ., et al. 2018. Angew Chem Int Ed Engl. 57: 8514-8518. PMID: 29718584
  3. Eosin Y- and Copper-Catalyzed Dark Reaction To Construct Ene-γ-Lactams.  |  Lei, WL., et al. 2018. Org Lett. 20: 7220-7224. PMID: 30403485
  4. Neutral-Eosin-Y-Photocatalyzed Silane Chlorination Using Dichloromethane.  |  Fan, X., et al. 2019. Angew Chem Int Ed Engl. 58: 12580-12584. PMID: 31310447
  5. Fentanyl Detection Using Eosin Y Paper Assays.  |  Canfield, JR., et al. 2020. J Forensic Sci. 65: 1432-1442. PMID: 32347988
  6. Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide.  |  Wang, J., et al. 2020. Anal Bioanal Chem. 412: 7595-7602. PMID: 32844280
  7. Studies of eosin Y - DNA interaction using a competitive binding assay.  |  Dukhopelnykov, E., et al. 2021. Spectrochim Acta A Mol Biomol Spectrosc. 247: 119114. PMID: 33166781
  8. Eosin Y-Catalyzed Synthesis of 3-Aminoimidazo[1,2-a]Pyridines via the HAT Process under Visible Light through Formation of the C-N Bond.  |  Singh, HK., et al. 2020. ACS Omega. 5: 29854-29863. PMID: 33251420
  9. Investigating eosin Y as a photocatalyst for the radical-dependent activity-based probing of deubiquitinating enzymes.  |  Taylor, NC. and McGouran, JF. 2021. Org Biomol Chem. 19: 2177-2181. PMID: 33630007
  10. Metal-Free Aminomethylation of Aromatic Sulfones Promoted by Eosin Y.  |  Thierry, T., et al. 2021. Chemistry. 27: 14826-14830. PMID: 34464004
  11. Exploring Eosin Y as a bimodular catalyst: organophotoacid mediated Minisci-type acylation of N-heteroarenes.  |  Roy, VJ., et al. 2022. Chem Commun (Camb). 58: 1776-1779. PMID: 35037922
  12. Eosin Y-Containing Metal-Organic Framework as a Heterogeneous Catalyst for Direct Photoactivation of Inert C-H Bonds.  |  Zhao, L., et al. 2022. Inorg Chem. 61: 7256-7265. PMID: 35507831
  13. Eosin-Y/Cu(OAc)2-catalyzed aerobic oxidative coupling reactions of glycine esters in the dark.  |  Chowdhury, R. 2022. Org Biomol Chem. 20: 5387-5392. PMID: 35748811
  14. Supported Eosin Y as a Photocatalyst for C-H Arylation of Furan in Batch and Flow.  |  Rossi, S., et al. 2022. Molecules. 27: PMID: 36014330
  15. Light-accelerated depolymerization catalyzed by Eosin Y.  |  Bellotti, V., et al. 2023. Polym Chem. 14: 253-258. PMID: 36760607

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Eosin Y, 5 g

sc-252776
5 g
$83.00

Eosin Y, 25 g

sc-252776A
25 g
$358.00

i would like to know how to work with eosin y to stain sperm cells in order to test viability. i get the reagent without instructions to use.

Asked by: zipi
Thank you for your question. We have not developed a specific protocol for using Eosin Y to stain sperm cells in order to test viability. I was able to find a reference to a publication in the scientific literature that does describe this protocol. PMID: 12660276. I have provided a link below to the paper on NCBI's PubMed. http://www.ncbi.nlm.nih.gov/pubmed/12660276
Answered by: Technical Support
Date published: 2016-09-19
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Rated 5 out of 5 by from Wang et alWang et al. (PubMed ID 26394744) used Eosin Y as a photosensitizer to compare H2 production activities in an artificial photosynthesis system. -SCBT Publication Review
Date published: 2015-05-04
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Eosin Y is rated 5.0 out of 5 by 1.
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