Anti-Endoglin/CD105 Antibody (SN6) recommended for detection of Endoglin cell surface antigen of human origin by WB, IP, IF, IHC(P) and FCM

Anti-Endoglin/CD105 Antibody (SN6): sc-57099

Anti-Endoglin/CD105 Antibody (SN6) is rated 3.0 out of 5 by 2.
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Datasheets

    • Endoglin/CD105 Antibody (SN6) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
    • raised against partially purified cell membrane antigens of human origin
    • recommended for detection of Endoglin cell surface antigen of human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Endoglin/CD105 (P3D1): sc-18838 for Endoglin/CD105 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Endoglin/CD105 Antibody (SN6). This work is in progress.
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Can Endoglin (SN6): sc-57099 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Endoglin (SN6): sc-57099 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Produced good immunoperoxidase cytoplasmic Produced good immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-02-25
Rated 3 out of 5 by from Produced good indirect FCM data for ECV Produced good indirect FCM data for ECV-304 cells stained with Endoglin . -SCBT QC
Date published: 2014-09-26
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