Anti-Endoglin/CD105 Antibody (P4A4) recommended for detection of Endoglin dimer under non-reducing conditions, and Endoglin monomer under reducing conditions of mouse, rat and human origin by WB, IP and IF

Anti-Endoglin/CD105 Antibody (P4A4): sc-20072

Anti-Endoglin/CD105 Antibody (P4A4) is rated 5.0 out of 5 by 8.
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Datasheets

    • Endoglin/CD105 Antibody (P4A4) is a mouse monoclonal IgG2b κ, cited in 13 publications, provided at 200 µg/ml
    • raised against Endoglin of human origin
    • Anti-Endoglin/CD105 Antibody (P4A4) is recommended for detection of Endoglin dimer under non-reducing conditions, and Endoglin monomer under reducing conditions of mouse, rat and human origin by WB, IP and IF
    • Anti-Endoglin/CD105 Antibody (P4A4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide free for activation of T cell binding to endothelium, sc-20072 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Endoglin/CD105 (P4A4): sc-20072.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Endoglin/CD105 Antibody (P4A4) for WB applications. These reagents are now offered in bundles with Endoglin/CD105 Antibody (P4A4) (see ordering information below).
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hello, do you guys have a CD105 antibody specific to canine?

Asked by: Orzala
Thank you for your question. This antibody has not yet been tested in canine and so that is not a recommended species at this time. We are still gathering data to show on our website. Feel free to reach out to Technical Service for further assistance
Answered by: Tech Service 1
Date published: 2021-02-04

I bought the L form of this antibody, sc-20072 L for blocking (neutralization). How long can I store this at in the refridgerator?

Asked by: Germaine
The L form of the antibody should be stored at -20°C. Once thawed, we recommend using as soon as possible, although it is usually stable for up to 2 weeks at 4 degrees.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IHC-P Immunofluorescence - Works Great Tested sample of this antibody for CD105 on IHC-P vascular tissue section following TE Buffer (pH 9.0) antigen retrieval (20 min, 95C). Staining was very specific with little background. Used anti-mouseAF488 secondary antibody at 1:500. Highly recommend this antibody for IHC-P immunofluorescence for Endoglin (CD105).
Date published: 2018-01-15
Rated 5 out of 5 by from Apelin抗体 我去年购买的此抗体SC-33804,内皮细胞WB结果显示该抗体特异性非常好,条带单一,大小在17KDa左右。
Date published: 2017-07-11
Rated 5 out of 5 by from Antibody was diluted 1:1000 in PBS Antibody was diluted 1:1000 in PBS
Date published: 2015-10-16
Rated 5 out of 5 by from IHC with paraffin embedded human cancer tissue IHC with paraffin embedded human cancer tissue specimens. -SCBT Publication Review
Date published: 2015-06-24
Rated 5 out of 5 by from Performed western blot with rat HSCs (hepatic Performed western blot with rat HSCs (hepatic stellate cells). -SCBT QC
Date published: 2015-05-27
Rated 5 out of 5 by from Produced superb immunofluorescence membrane Produced superb immunofluorescence membrane staining in methanol-fixed HUV-EC-C cells. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Strong band in western blot using human recombinant Strong band in western blot using human recombinant Endoglin. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Positive result in western blot analysis Positive result in western blot analysis of Endoglin expression in mouse embryo tissue extract -SCBT QC
Date published: 2015-01-12
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