Anti-Endoglin/CD105 Antibody (P3D1) recommended for detection of TGF-Receptor III complex (known also as Endoglin or CD105) of Endoglin of mouse, rat and human origin by WB, IP, IF and FCM

Anti-Endoglin/CD105 Antibody (P3D1): sc-18838

Anti-Endoglin/CD105 Antibody (P3D1) is rated 4.5 out of 5 by 15.
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Datasheets

    • Anti-Endoglin/CD105 Antibody (P3D1) is a mouse monoclonal IgG2a κ Endoglin/CD105 antibody, cited in 29 publications, provided at 200 µg/ml
    • raised against Endoglin of human origin
    • Anti-Endoglin/CD105 Antibody (P3D1) is recommended for detection of TGF-Receptor III complex (known also as Endoglin or CD105) of Endoglin of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-Endoglin/CD105 Antibody (P3D1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Endoglin/CD105 (P3D1): sc-18838.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Endoglin/CD105 Antibody (P3D1). This work is in progress.
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Has anyone tested this against Rat cells since it is recommended to work in rats too?

Asked by: Neha Rana
Thank you for your question. Yes, this antibody is rat reactive and it is recommended and warrantied for that use.
Answered by: Tech Service
Date published: 2019-04-16

Has anyone checked this antibody against Rat mesenchymal stem cells ?

Asked by: Neha Rana
Thank you for your question. Unfortunately we are not aware of any use specifically on rat mesenchymal stem cells, but we do know the antibody is rat reactive.
Answered by: Tech Service
Date published: 2019-04-16

What is the emission wavelength of this antibody(sc-18838 AF647)?

Asked by: yuzhizhi
Thank you for your question. The excitation for AF647 is 652nm and the emission is 668nm.
Answered by: Tech Service
Date published: 2019-01-17

I've tested a different protocol for IHC-P, but tonsil tissue as the positive control is not stained. my concern is about storage condition, I stored it in -20 for 2 months, can these storage conditions be problematic?

Asked by: SAHAR
Thank you for your question. Most of our antibodies are provided in PBS with gelatin and sodium azide. When the antibody is frozen, it precipitates out of the solution due to the presence of gelatin and may denature. We recommend storing all antibodies contained in PBS with gelatin at 4 °C. At this temperature, they are usually viable for a number of years.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: cjMara
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-02-02
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Rated 5 out of 5 by from Works great for ICC in human BMMSCs and ADMSCs Bought this two months ago and it has worked perfectly with our human mesenchymal stem cells. It worked with dilutions from 1:50-1:200
Date published: 2020-03-10
Rated 1 out of 5 by from 蛍光免疫染色 4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いましたが特異的なシグナルは得られませんでした。
Date published: 2018-11-26
Rated 4 out of 5 by from good use dilution 1: 150 over night for immunofluoresce
Date published: 2018-04-10
Rated 4 out of 5 by from good we use sc-18838 to detect stem cells in human intervertebral disc tissues at a concentration of 1:50, some cells were labeled.
Date published: 2017-12-14
Rated 4 out of 5 by from work good we've got a positve results in IHC after applicate it into hPDLCs , but the results is not what we needed
Date published: 2017-05-31
Rated 5 out of 5 by from positive Positive signal in WB in mouse embryo tissue, very stable antibody
Date published: 2017-05-07
Rated 5 out of 5 by from great features I bought the antibody a mouth ago,and the product shows good quality for western blot with human colon cancer cells detection,used this several times and get very clean bands
Date published: 2017-05-02
Rated 5 out of 5 by from perfect result We used this antibody to detect 1:800 cell lines from WB, the antibody have a good stability and specificity.
Date published: 2017-04-07
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