Anti-Endoglin/CD105 Antibody (MJ7/18) recommended for detection of Endoglin of mouse, rat and human origin by WB, IP, IF and FCM

Anti-Endoglin/CD105 Antibody (MJ7/18): sc-18893

Anti-Endoglin/CD105 Antibody (MJ7/18) is rated 3.3 out of 5 by 6.
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Datasheets

    • Endoglin/CD105 Antibody (MJ7/18) is a rat monoclonal IgG2a, cited in 11 publications, provided at 200 µg/ml
    • raised against inflamed mouse skin tissue
    • recommended for detection of Endoglin of mouse, rat and human origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Endoglin/CD105 (P3D1): sc-18838 for Endoglin/CD105 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Endoglin/CD105 Antibody (MJ7/18). This work is in progress.
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Can Endoglin (MJ7/18): sc-18893 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Endoglin (MJ7/18): sc-18893 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WB with mouse kidney extracts WB with mouse kidney extracts. -SCBT Publication Review
Date published: 2015-05-27
Rated 3 out of 5 by from IF with murine uterus sections IF with murine uterus sections. -SCBT Publication Review
Date published: 2015-05-26
Rated 4 out of 5 by from Published Flow Cytometry data using murine Published Flow Cytometry data using murine embryonic endothelial cells. -SCBT Publication Review
Date published: 2015-05-16
Rated 3 out of 5 by from Decent immunofluorescence membrane staining Decent immunofluorescence membrane staining in methanol-fixed JAR cells. -SCBT QC
Date published: 2015-03-31
Rated 3 out of 5 by from Used for immunohistochemical analysis of Used for immunohistochemical analysis of paraffin embedded human testes sections. -SCBT Publication Review
Date published: 2015-02-24
Rated 3 out of 5 by from Satisfactory FCM data of mouse peripheral Satisfactory FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-03-15
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