Anti-Endoglin/CD105 Antibody (8E11) recommended for detection of Endoglin of human origin by WB, IP and FCM

Anti-Endoglin/CD105 Antibody (8E11): sc-53999

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Anti-Endoglin/CD105 Antibody (8E11) is rated 2.0 out of 5 by 1.
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Datasheets

    • Endoglin/CD105 Antibody (8E11) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
    • raised against monocytic U937 phorbol ester-treated cells of human origin
    • recommended for detection of Endoglin of human origin by WB, IP and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See Endoglin/CD105 (P3D1): sc-18838 for Endoglin/CD105 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Endoglin/CD105 Antibody (8E11) for WB applications. This reagent is now offered in a bundle with Endoglin/CD105 Antibody (8E11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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How can Endoglin (8E11): sc-53999 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Randy McDonald
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53999 PE) or fluoroscein (sc-53999 FITC).
Answered by: Technical Support
Date published: 2017-02-25
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