Electrophoresis Sample Buffer, 2X A ready-to-use reducing sample buffer for SDS-PAGE.

Electrophoresis Sample Buffer, 2X

Electrophoresis Sample Buffer, 2X is rated 4.4 out of 5 by 8.
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Alternate Names: Electrophoresis Sample Buffer, 2X is known as the most common SDS-PAGE sample buffer.
Application: Electrophoresis Sample Buffer, 2X is a ready-to-use reducing sample buffer for SDS-PAGE, acting on denatured proteins in the system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included with Electrophoresis Sample Buffer, 2X as a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer, 2X contains DTT (sc-29089), which disrupts disulfide bonds to ensure proteins are fully denatured before loading on the gel. Electrophoresis Sample Buffer, 2X also contains glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue. For non-reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (non-reducing) (sc-45085) is recommended.


References

1. Toyoda, E. et al. 2005. Pancreas. 30(2): 168-173. PMID: 15714139
2. Heinz, L.X. et al. 2006. Blood. 107(4): 1445-1453. PMID: 16223775
3. Gohil, K. et al. 2008. Brain Res. 1201 : 167-176. PMID: 18299118
4. Popovici, R.M. et al. 2008. Endocrinology. 149(3): 1136-1143. PMID: 18048494
5. Tang, Y. et al. 2010. J. Nutr. Biochem. 21(3): 237-246. PMID: 19369054

Usage :
Mix sample with an equal volume of Electrophoresis Sample Buffer, 2X. Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
Appearance :
Aqueous solution
Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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Electrophoresis Sample Buffer, 2X  Product Citations

See how others have used Electrophoresis Sample Buffer, 2X. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 32 total

PMID: # 34142127  Rios, JJ.|Denton, K.|Yu, H.|Manickam, K.|Garner, S.|Russell, J.|Ludwig, S.|Rosenfeld, JA.|Liu, P.|Munch, J.|Sucato, DJ.|Beutler, B.|Wise, CA.| et al. 2021. Dis Model Mech. 14:

PMID: # 34079997  Rios, JJ.|Denton, K.|Yu, H.|Manickam, K.|Garner, S.|Russell, J.|Ludwig, S.|Rosenfeld, JA.|Liu, P.|Munch, J.|Sucato, DJ.|Beutler, B.|Wise, CA.| et al. 2021. Dis Model Mech.

PMID: # 29326957  Garrido, D. et al. 2017. Front Vet Sci. 4: 226.

PMID: # 26514550  Winiarska, K. et al. 2016. Journal of pineal research. 60: 109-17.

PMID: # 26432891  Talaei, N. et al. 2015. J. Immunol. 195: 4623-31.

PMID: # 26734660  Peng, S. et al. 2015. Annals of clinical and translational neurology. 2: 1085-104.

PMID: # 24370615  Joiner, DM. et al. 2014. Calcified tissue international. 94: 484-94.

PMID: # 23686888  Jia, Y. et al. 2013. Andrology. 1: 651-9.

PMID: # 23787122  Kaufman, JL. et al. 2013. Clinical lymphoma, myeloma & leukemia. 13: 370-6.

PMID: # 23291594  Wang, R. et al. 2013. Free radical biology & medicine. 57: 141-53.

Citations 1 to 10 of 32 total

沈殿物があり、転倒混和しても溶解しません。また浮遊している粒子がピペットで吸うときに詰まって、正確な量が吸えません。 粒子は沈殿させて上清を吸えば、製品として問題ないのでしょうか?ご教示ください。

Asked by: naganuma1031
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2017-10-27
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Rated 5 out of 5 by from Easy lysis and sample prep in one step This reagent is easy to use and provided complete lysis of our cells. It is very convenient to have the lysate in a sample buffer, ready to load on a gel.
Date published: 2021-07-22
Rated 5 out of 5 by from Convenient to use Very convenient since DTT is already included in the solution. Saves time in western blot preparation
Date published: 2019-02-04
Rated 5 out of 5 by from The best one Really good one. I would definitely go for it again.
Date published: 2018-09-24
Rated 2 out of 5 by from Disappointed I am unhappy with this product because of 2 reasons: The products gets precipitated and difficult to pipette . The dye concentration is too low making it difficult to track the protein sample.
Date published: 2018-07-04
Rated 5 out of 5 by from Electrophoresis sample buffer Great sample buffer for protein gel electrophoresis, provided nice results.
Date published: 2018-03-28
Rated 5 out of 5 by from Very clear we use it detect the α E-catenin in cancer cell, we usually use the dilution 1:800, bands came out very clear.
Date published: 2017-04-29
Rated 5 out of 5 by from Results were great. Electrophoresis Buffer antibody provides clear, specific results and good specificity. On the whole, we are satisfied with the antibody.
Date published: 2017-02-07
Rated 3 out of 5 by from Precipitation occurs Precipitation occurs
Date published: 2015-04-03
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