eIF2B delta Antibody (P-6)recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and IHC(P)

eIF2Bδ Antibody (P-6): sc-9981

eIF2Bδ Antibody (P-6) is rated 4.5 out of 5 by 4.
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Datasheets

    • eIF2Bδ Antibody (P-6) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against full length eIF2Bδ
    • eIF2B delta Antibody (P-6) is recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • eIF2B delta Antibody (P-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of eIF2Bδ (P-6): sc-9981.
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Can eIF2Bδ (P-6): sc-9981 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, eIF2Bδ (P-6): sc-9981 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Good cytoplasmic localization of IF staining Good cytoplasmic localization of IF staining with methanol-fixed KNRK cells -SCBT QC
Date published: 2015-04-04
Rated 4 out of 5 by from Works well in IHC with paraffin Works well in IHC with paraffin-embedded human smooth muscle tissue -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Very strong bands observed in WB with KNRK and K Very strong bands observed in WB with KNRK and K-562 whole cell lysates, some background visible -SCBT QC
Date published: 2014-09-11
Rated 4 out of 5 by from human samples human samples
Date published: 2003-03-17
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