eIF2B delta Antibody (H-4)recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and ELISA

eIF2Bδ Antibody (H-4): sc-271795

eIF2Bδ Antibody (H-4) is rated 4.7 out of 5 by 3.
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Datasheets

    • eIF2Bδ Antibody (H-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 224-503 mapping near the C-terminus of eIF2Bδ of human origin
    • recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with eIF2Bδ (H-4): sc-271795 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Use it for Western Blot (1:500) detecting Use it for Western Blot (1:500) detecting total protein lysates (20 micro-gram) from mouse embryonic fibroblast cell line. Very specific (one band only) and reasonably strong. Size is accurate, slightly above 55 Kd marker. I am satisfied with this antibody.
Date published: 2015-10-19
Rated 5 out of 5 by from Cytoplasmic localization of the eIF2B protein Cytoplasmic localization of the eIF2B protein shown by immunofluorescence of methanol fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Robust signal obtained by Western Blot using K Robust signal obtained by Western Blot using K-562, KNRK and ES-2 whole cell lysates. Clean background. -SCBT QC
Date published: 2013-02-28
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