EHD1 Double Nickase Plasmid (h): sc-407060-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EHD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- EHD1 Double Nickase Plasmid (h) and EHD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EHD1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EHD1 Double Nickase Plasmid (h) | sc-407060-NIC | 20 µg | $410.00 | |||
EHD1 Double Nickase Plasmid (h2) | sc-407060-NIC-2 | 20 µg | $410.00 |
EHD1 (EH domain containing 1) encodes an ATPase of the EHD protein family that regulates endocytic recycling and membrane remodeling, coordinating return of receptors and lipids from recycling endosomes to the plasma membrane. Through interactions with phospholipids and endocytic adaptors, EHD1 supports clathrin-associated trafficking, tubular endosome formation, and receptor recycling programs that influence signaling amplitude and cell migration. EHD1-dependent trafficking impacts pathways linked to nutrient uptake and growth factor receptor turnover, including transferrin receptor and EGFR recycling dynamics. Dysregulated EHD1 expression or function has been associated in the literature with altered invasive behavior and signaling rewiring in cancer models, as well as perturbations in epithelial polarity and neuronal membrane trafficking relevant to neurobiology.
EHD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EHD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EHD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EHD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EHD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.