EF-1 alpha 1/2 Antibody (G-8)recommended for detection of EF-1 α1 and EF-1 α2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

EF-1 α1/2 Antibody (G-8): sc-377439

EF-1 α1/2 Antibody (G-8) is rated 4.7 out of 5 by 3.
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Datasheets

    • EF-1 α1/2 Antibody (G-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 163-462 mapping at the C-terminus of EF-1 α1 of human origin
    • EF-1 alpha 1/2 Antibody (G-8) is recommended for detection of EF-1 α1 and EF-1 α2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • EF-1 alpha 1/2 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EF-1 α1/2 (G-8): sc-377439.
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Can EF-1 α1/2 (G-8): sc-377439 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, EF-1 α1/2 (G-8): sc-377439 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from good with 1:1000 dilution ,the band is clear,for western blotting,this antibody is OK
Date published: 2017-02-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human hippocampus tissue. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Satisfactory Western Blot data of EF Satisfactory Western Blot data of EF-1 1/2 expression in HL-60, Hep G2, U-87 MG, MOLT-4 and A549 whole cell lysates. -SCBT QC
Date published: 2014-03-14
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