Ebi3 Antibody (G-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
raised against amino acids 154-228 mapping at the C-terminus of Ebi3 of mouse origin
Ebi3 Antibody (G-4) is recommended for detection of Ebi3 of mouse, rat and human origin by WB, IP, IF and ELISA
Ebi3 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ebi3 (G-4): sc-166158.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 3 out of
Antibody reviewThis antibody gave me a faint band in Immunoblotting at 1:500 dilution with mouse tissue lysate.
Date published: 2018-06-14
Rated 4 out of
EBI3 antibody (G-4)We have tried this antibody for Western blot, it can detect EBI3 in cell lysate, but with some non-specific bands, which may be due to secondary antibody used in our WB. I wonder whether we can have a try for your HRP conjugated EBI3 antibody (G-4). We also tried coating this antibody to detect EBI3 in ELISA, it seems works as a capture antibody, the specificity was not great. I wonder I could use HRP-conjugated EBI3 antibody (G-4) as detecting antibody for ELISA? It will be great if you can kindly send me HRP-conjugated EBI3 antibody for a trial study. Many thanks.
Date published: 2018-04-11
Rated 5 out of
Produced publishable data in mouseThis Ebi3 monoclonal was referenced in the primary literature used on mouse corneal epithelial cells.
Date published: 2017-01-20
Rated 5 out of
Robust band observed in WB in IRobust band observed in WB in I-11.15 whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of
Clean blot with single strong band at expectedClean blot with single strong band at expected size using RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-01-25
Rated 5 out of
Good specificity and signal strength in mouseGood specificity and signal strength in mouse Ebi3 transfected 293T whole cell lysate. -SCBT QC
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