Anti-Ebi3 Antibody (G-4) is a mouse monoclonal IgG2a (kappa light chain) Ebi3 antibody provided at 200 µg/ml
raised against amino acids 154-228 mapping at the C-terminus of Ebi3 of mouse origin
Anti-Ebi3 Antibody (G-4) is recommended for detection of Ebi3 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-Ebi3 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ebi3 (G-4): sc-166158.
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Labware ships FedEx Ground free of charge to the contiguous US
Ebi3 Antibody (G-4) is a high quality monoclonal Ebi3 antibody (also designated Ebi3 antibody) suitable for the detection of the Ebi3 protein of mouse, rat and human origin. Ebi3 Antibody (G-4) is available as both the non-conjugated anti-Ebi3 antibody form, as well as multiple conjugated forms of anti-Ebi3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 3 out of
Antibody reviewThis antibody gave me a faint band in Immunoblotting at 1:500 dilution with mouse tissue lysate.
Date published: 2018-06-14
Rated 4 out of
EBI3 antibody (G-4)We have tried this antibody for Western blot, it can detect EBI3 in cell lysate, but with some non-specific bands, which may be due to secondary antibody used in our WB. I wonder whether we can have a try for your HRP conjugated EBI3 antibody (G-4). We also tried coating this antibody to detect EBI3 in ELISA, it seems works as a capture antibody, the specificity was not great. I wonder I could use HRP-conjugated EBI3 antibody (G-4) as detecting antibody for ELISA? It will be great if you can kindly send me HRP-conjugated EBI3 antibody for a trial study. Many thanks.
Date published: 2018-04-11
Rated 5 out of
Produced publishable data in mouseThis Ebi3 monoclonal was referenced in the primary literature used on mouse corneal epithelial cells.
Date published: 2017-01-20
Rated 5 out of
Robust band observed in WB in IRobust band observed in WB in I-11.15 whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of
Clean blot with single strong band at expectedClean blot with single strong band at expected size using RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-01-25
Rated 5 out of
Good specificity and signal strength in mouseGood specificity and signal strength in mouse Ebi3 transfected 293T whole cell lysate. -SCBT QC
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