Ebi3 Antibody (G-4)recommended for detection of Ebi3 of mouse, rat and human origin by WB, IP, IF and ELISA

Ebi3 Antibody (G-4): sc-166158

Ebi3 Antibody (G-4) is rated 4.5 out of 5 by 6.
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Datasheets

    • Ebi3 Antibody (G-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 154-228 mapping at the C-terminus of Ebi3 of mouse origin
    • Ebi3 Antibody (G-4) is recommended for detection of Ebi3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Ebi3 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ebi3 (G-4): sc-166158.
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-166158: Ebi3 (G-4) mouse monoclonal antibody will work?

Asked by: Trav11
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 3 out of 5 by from Antibody review This antibody gave me a faint band in Immunoblotting at 1:500 dilution with mouse tissue lysate.
Date published: 2018-06-14
Rated 4 out of 5 by from EBI3 antibody (G-4) We have tried this antibody for Western blot, it can detect EBI3 in cell lysate, but with some non-specific bands, which may be due to secondary antibody used in our WB. I wonder whether we can have a try for your HRP conjugated EBI3 antibody (G-4). We also tried coating this antibody to detect EBI3 in ELISA, it seems works as a capture antibody, the specificity was not great. I wonder I could use HRP-conjugated EBI3 antibody (G-4) as detecting antibody for ELISA? It will be great if you can kindly send me HRP-conjugated EBI3 antibody for a trial study. Many thanks.
Date published: 2018-04-11
Rated 5 out of 5 by from Produced publishable data in mouse This Ebi3 monoclonal was referenced in the primary literature used on mouse corneal epithelial cells.
Date published: 2017-01-20
Rated 5 out of 5 by from Robust band observed in WB in I Robust band observed in WB in I-11.15 whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using RAW 264.7 whole cell lysate. -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from Good specificity and signal strength in mouse Good specificity and signal strength in mouse Ebi3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2014-08-04
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