EBF Antibody (C-8)recommended for detection of EBF1, EBF2, EBF3 and EBF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EBF Antibody (C-8): sc-137065

EBF Antibody (C-8) is rated 4.8 out of 5 by 9.
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Datasheets

    • EBF Antibody (C-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of EBF of human origin
    • EBF Antibody (C-8) is recommended for detection of EBF1, EBF2, EBF3 and EBF4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • EBF Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EBF (C-8): sc-137065.
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I am using EBF (C-8): sc-137065 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from Excellent for IF Test IF (1:1000 with antigen retrieval) on frozen section from 4%PFA fixed embryonic mouse brain. It gives clear, specific signal.
Date published: 2019-01-03
Rated 4 out of 5 by from Working EBF antibody for WB Working EBF antibody for WB. Clean background with one or two non-specific bands.
Date published: 2017-10-18
Rated 4 out of 5 by from Verified in Ebf1 KO sample Ebf1 KO mouse brain tissue lysates. There is one band deficiency at around 63 kDa in Ebf1-/- sample. But there are many bands.
Date published: 2017-09-29
Rated 5 out of 5 by from Great I ordered this antibody and test this antibody with overexpressing EBF1 and endogenous protein. It work great! I got the specific and clear band.
Date published: 2017-08-14
Rated 5 out of 5 by from Awesome antibody EBF Antibody (C-8) is great for WB and IHC! It produced positive and specific signal
Date published: 2017-01-21
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using Ramos and IMR-32 whole cell lysates. -SCBT QC
Date published: 2015-07-17
Rated 5 out of 5 by from Band detected at expected mw in human EBF1 Band detected at expected mw in human EBF1 transfected 293T whole cell lysates and Ramos nuclear extract. -SCBT QC
Date published: 2015-07-06
Rated 5 out of 5 by from ChIP publishable data with human GM12878 cells ChIP publishable data with human GM12878 cells - SCBT Publication Review
Date published: 2015-05-01
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