EB1 Antibody (F-7)recommended for detection of EB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

EB1 Antibody (F-7): sc-374474

EB1 Antibody (F-7) is rated 5.0 out of 5 by 5.
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Datasheets

    • EB1 Antibody (F-7) is a mouse monoclonal IgA (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 239-268 at the C-terminus of EB1 of human origin
    • recommended for detection of EB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • blocking peptide, sc-374474 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See EB1 (1A11/4): sc-47704 for EB1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What application is the blocking peptide sc-374474 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from i have performed Immunohistochemistry and i have performed Immunohistochemistry and western blotting in oral cancer tissue samples as well as immunofluoresence in oral cancer cell line. It works well.
Date published: 2015-10-31
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Robust signal in WB in HeLa whole cell lysate Robust signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Strong band in WB in SW480 whole cell lysate Strong band in WB in SW480 whole cell lysate. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Good specificity in WB in COLO320DM and HISM Good specificity in WB in COLO320DM and HISM whole cell lysates. -SCBT QC
Date published: 2015-01-10
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