E-cadherin Antibody (SPM471) recommended for detection of E-cadherin of human origin by WB, IP, IF and IHC(P)

E-cadherin Antibody (SPM471): sc-56527

E-cadherin Antibody (SPM471) is rated 4.2 out of 5 by 5.
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Datasheets

    • E-cadherin Antibody (SPM471) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 600-707 of E-cadherin of human origin
    • recommended for detection of E-cadherin of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of E-cadherin (SPM471): sc-56527.
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What is the recommended starting dilution for Western blot for sc-56527: E-cadherin (SPM471) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good for western blot I asked for a tube of free sample and tested it on prostate cancer cells. Then it works great and show the exact one band at around 130 KD, although I am not hundred percent sure that this band is E-Cadherin. No unspecific background.
Date published: 2019-05-09
Rated 4 out of 5 by from MCF7細胞の免疫染色を行えます。 サンプルを頂いてMCF7細胞の免疫染色を行いました。問題なく染色できました。なお、固定方法は4%パラホルムアルデヒドです。
Date published: 2019-04-17
Rated 5 out of 5 by from Worked really well for MCF-7 cell E-Cadherin WB Worked really well for MCF-7 cell E-Cadherin WB @ 1 :500
Date published: 2018-08-28
Rated 4 out of 5 by from Good product It is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
Rated 3 out of 5 by from Showed target bands in LNCaP whole cell lysates Showed target bands in LNCaP whole cell lysates in WB, some additional banding -SCBT QC
Date published: 2013-10-04
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