Dynein IC1/2, cytosolic Antibody (74-1)recommended for detection of intermediate chains 1 and 2 of cytosolic Dynein of broad species origin by WB, IP, IF and FCM

Dynein IC1/2, cytosolic Antibody (74-1): sc-13524

Dynein IC1/2, cytosolic Antibody (74-1) is rated 5.0 out of 5 by 10.
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Datasheets
    • Dynein IC1/2, cytosolic Antibody (74-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against purified cytosolic dynein from brain tissue of bovine origin
    • recommended for detection of intermediate chains 1 and 2 of cytosolic Dynein of broad species origin by WB, IP, IF and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dynein IC1/2, cytosolic (74-1): sc-13524.
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What is the recommended secondary for this mouse monoclonal primary?

Asked by: AbPolly
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-20

Can this antibody be used with Xenopus samples?

Asked by: AbPolly
This antibody is recommended for use with a broad range of species. For specific species, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data.
Answered by: Technical Support 14
Date published: 2019-03-04
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Rated 5 out of 5 by from Good for IF This Dynein IC1/2, cytosolic Antibody (74-1), sc-13524, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from Works well for Western Blot We used this antibody in CV-1(monkey) cell line after performing a western blot. 1:2000 in milk. The bands were clear with very little background.
Date published: 2017-11-01
Rated 5 out of 5 by from Great product Outstanding product for both western blot and IF staining applications
Date published: 2017-01-20
Rated 5 out of 5 by from Strong signal This Dynein IC1/2, cytosolic antibody shows strong signal in WB.
Date published: 2017-01-12
Rated 5 out of 5 by from Great in WB!!! Detects a single band in HeLa lysate at a 1:500 dilution. VERY strong single.
Date published: 2016-11-29
Rated 5 out of 5 by from Positive western blot detection of Dynein IC1/2 Positive western blot detection of Dynein IC1/2, cytosolic expression in T98G, IMR-32 and L8 whole cell lysates. -SCBT QC
Date published: 2015-07-23
Rated 5 out of 5 by from Great intracellular FCM data of fixed and Great intracellular FCM data of fixed and permeabilized L8 cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed T98G cells. -SCBT QC
Date published: 2015-03-29
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