Dynein HC Antibody (C-5)recommended for detection of Dynein HC of mouse, rat and human origin by WB, IP, IF and ELISA

Dynein HC Antibody (C-5): sc-514579

Dynein HC Antibody (C-5) is rated 4.8 out of 5 by 6.
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Datasheets
    • Dynein HC Antibody (C-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 4320-4644 of Dynein HC of rat origin
    • Dynein HC Antibody (C-5) is recommended for detection of Dynein HC of mouse, rat and human origin by WB, IP, IF and ELISA
    • Dynein HC Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dynein HC (C-5): sc-514579.
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I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-514579: Dynein HC (C-5) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Good for IF This Dynein HC Antibody (C-5), sc-514579, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from Works well for WB and IP We used this antibody for both WB (1:2000 in milk) and IP. The antibody worked well in both applications using a monkey (CV-1) cell line.
Date published: 2017-11-01
Rated 5 out of 5 by from Specific antibody I enjoy the specific binding I get by immunoblot with this antibody.
Date published: 2017-10-03
Rated 4 out of 5 by from Works in IF Works well in IF although a little weak. I used it in PFA fixed human colon carcinoma cells. Dilution 1:100.
Date published: 2017-09-27
Rated 5 out of 5 by from worked Well The antibody worked well. I have used 3-8% bis tris gels and a LICOR method of detection.
Date published: 2017-06-08
Rated 5 out of 5 by from Great in WB Works great in WB at a 1:500 dilution using HeLa cell lysate
Date published: 2016-12-06
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