Dynamin I Antibody (D5)recommended for detection of Dynamin I of mouse, rat, human, canine and avian origin by WB, IP and IF

Dynamin I Antibody (D5): sc-12724

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Datasheets

    • Dynamin I Antibody (D5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-750 of Dynamin I of human origin
    • Dynamin I Antibody (D5) is recommended for detection of Dynamin I of mouse, rat, human, canine and avian origin by WB, IP and IF
    • Dynamin I Antibody (D5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dynamin I (D5): sc-12724.
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What is the recommended fixation method for immunofluorescence staining with Dynamin I (D5): sc-12724 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2019, m_12, d_5, h_17CST
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Rated 5 out of 5 by from very well working antibody Got clear bands with sc-12724 sc mouse monoclonal antibody in rat heart samples
Date published: 2017-03-16
Rated 5 out of 5 by from Robust signal in WB in human Dynamin I transfected Robust signal in WB in human Dynamin I transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-07
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmic Produced superb immunofluorescence cytoplasmic staining in methanol-fixed SK-N-SH cells. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Published data for WB in human 293 FT and Published data for WB in human 293 FT and HeLa cells. -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from Published IHC(P) data for use in monkey brain Published IHC(P) data for use in monkey brain. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Good Good, strong band observed in WB in rat brain and mouse brain extracts and SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Published data for CoIP and WB in human H295R Published data for CoIP and WB in human H295R adrenocortical cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Worked great in WB in SH Worked great in WB in SH-SY5Y whole cell lysate and human cerebral cortex tissue extract. -SCBT QC
Date published: 2015-01-26
  • y_2019, m_12, d_5, h_17
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