Anti-Dok-1 Antibody (A-3) recommended for detection of Dok-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Dok-1 Antibody (A-3): sc-6929

Anti-Dok-1 Antibody (A-3) is rated 4.8 out of 5 by 12.
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Datasheets

    • Anti-Dok-1 Antibody (A-3) is a mouse monoclonal IgG1 κ Dok-1 antibody, cited in 18 publications, provided at 200 µg/ml
    • raised against amino acids 1-276 mapping at the N-terminus of Dok-1 of mouse origin
    • Anti-Dok-1 Antibody (A-3) is recommended for detection of Dok-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Dok-1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dok-1 (A-3): sc-6929.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dok-1 Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Dok-1 Antibody (A-3) (see ordering information below).
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I am using Dok-1 (A-3): sc-6929 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Well-rounded antibody for Dok-1! Very strong and specific for Dok-1 detection on IHC on paraffin tissue sections. Also really good for Western blot use. Great choice!
Date published: 2017-01-30
Rated 5 out of 5 by from Great antibody for Western i bought this antibody a month ago and used successfully in Western with 3T3-L1 cells
Date published: 2016-12-07
Rated 5 out of 5 by from Used successfully in WB and IHC Used successfully in WB and IHC. Published - See PubMed ID 21865257.
Date published: 2015-09-29
Rated 4 out of 5 by from Decent stains in Western achieved using mouse Dok Decent stains in Western achieved using mouse Dok-1 transfected 293T whole cell lysate.
Date published: 2015-07-19
Rated 5 out of 5 by from Published data for IP and WB in mouse J 1 Published data for IP and WB in mouse J 1 ES and B cells -SCBT QC
Date published: 2015-07-10
Rated 5 out of 5 by from Superb immunoperoxidase nuclear staining Superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-05-25
Rated 5 out of 5 by from WB positive with mouse NIH//3T3 cells WB positive with mouse NIH//3T3 cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Publishable data in coimmunoprecipitation Publishable data in coimmunoprecipitation and Western blotting with this antibody in GC cell line and HEK293 -SCBT Publication Review
Date published: 2015-03-02
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