Anti-Dok-1 Antibody (45) recommended for detection of Dok-1 of human origin by WB

Anti-Dok-1 Antibody (45): sc-135888

Anti-Dok-1 Antibody (45) is rated 4.0 out of 5 by 1.
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Datasheets

    • Dok-1 Antibody (45) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 331-478 of Dok-1 of human origin
    • recommended for detection of Dok-1 of human origin by WB; not recommended for IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Dok-1 Antibody (45). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using Dok-1 (45): sc-135888?

Asked by: Dr Ninau Qelp
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Strong band seen in WB using Hs68 whole cell Strong band seen in WB using Hs68 whole cell lysate. -SCBT QC
Date published: 2013-04-07
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