DOHH Antibody (E-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-302 representing full length DOHH of human origin
DOHH Antibody (E-2) is recommended for detection of DOHH of mouse, rat and human origin by WB, IP, IF and ELISA
DOHH Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DOHH (E-2): sc-271868.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
DOHH (deoxyhypusine hydroxylase/monooxygenase), also known as HLRC1 (HEAT-like (PBS lyase) repeat containing 1), is a metalloenzyme involved in hypusine synthesis. It contains eight tandem HEAT-repeats, four at the N-terminus and four at the C-terminus. DOHH is an important player in mediating the posttranslational modifications of eIF5a to form hypusine. The first step of this reaction is catalyzed by DHS (deoxyhypusine synthase), which is responsible for transferring the aminobutyl moiety of spermidine to a lysine residue of eIF5a to form a deoxyhypusine-containing eIF5a intermediate. DOHH catalyzes the second step, hydroxylating the intermediate to form the hypusine residue thereby activating eIF5a. DHS, DOHH and eIF5a are evolutionarily conserved proteins that are essential for cell proliferation. Inhibition of DOHH can result in cell cycle arrest at the G1/S boundary. This suggests a potential use of DOHH inhibitors in antitumor therapy.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with DOHH (E-2): sc-271868 monoclonal antibody?
Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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