Dnmt3a Antibody (C-12)recommended for detection of Dnmt3a of mouse, rat and human origin by WB, IP and ELISA

Dnmt3a Antibody (C-12): sc-365769

Dnmt3a Antibody (C-12) is rated 4.3 out of 5 by 12.
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Datasheets

    • Dnmt3a Antibody (C-12) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 42-67 near the N-terminus of Dnmt3a of human origin
    • Dnmt3a Antibody (C-12) is recommended for detection of Dnmt3a of mouse, rat and human origin by WB, IP and ELISA
    • Dnmt3a Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-365769 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dnmt3a (C-12): sc-365769.
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Can I use the anti-Dnmt3a-AF647 antibody ( sc-365769 AF647) for flow cytometry? If so, do you have a recommended starting point for the concentration to be used?

Asked by: Akp1
Thank you for your question. sc-365769 AF647 has not been tested for flow cytometry in house, so we do not warranty it for that use, however it may work perfectly well. We recommend using ~1µg per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-26

I want to run a Western blot using this Dnmt3a antibody using NIH/3T3 cell lysate. How much should I load on my gel?

Asked by: TinTin
We recommend loading ~50µg of cell lysate on your gel. You can find more about the recommended conditions in our Western blotting protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Service 11
Date published: 2017-01-19
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Rated 4 out of 5 by from Work well! 1:200 diluted for wb of rat protein,the band is strong
Date published: 2018-03-19
Rated 4 out of 5 by from The band is clear,but there are other bands 1:200 diluted for wb of rat pretein,the band is clear,but there are other bands
Date published: 2018-02-13
Rated 5 out of 5 by from 抗体很好用 我在人源正常细胞、人源以及鼠源肿瘤细胞中进行Western blot检测,皆能很好的杂出清晰唯一的条带,实验效果很棒!
Date published: 2017-11-27
Rated 2 out of 5 by from Scarsa specificità In figura è mostrato il WB contro DNMT3A su lisato della linea cellulare di Neuroblastoma SH-SY5Y
Date published: 2017-11-20
Rated 4 out of 5 by from a good antibody for WB This antibody recognizes a band at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate). However, it does not appear to stain the nuclei in formaldehyde-fixed HeLa cells
Date published: 2017-07-30
Rated 5 out of 5 by from Superb Great product for western blot studies; also strong IHC staining results
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Dnmt3a expression in NIH/3T3 and Jurkat whole cell lysates.
Date published: 2017-01-12
Rated 4 out of 5 by from Monoclonal antibodies I tried DNMT3A mAb (C12) and I am convinced with that..
Date published: 2016-12-19
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