Dnmt3a Antibody (A-10)recommended for detection of Dnmt3a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Dnmt3a Antibody (A-10): sc-373905

Dnmt3a Antibody (A-10) is rated 5.0 out of 5 by 4.
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Datasheets

    • Dnmt3a Antibody (A-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-295 of Dnmt3a of human origin
    • Dnmt3a Antibody (A-10) is recommended for detection of Dnmt3a of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Dnmt3a Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dnmt3a (A-10): sc-373905.
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Have you tested if anti-Dnmt3a (A-10) or anti-Dnmt3a (C-12) detects Pig Dnmt3A (endogenous) by WB ?

Asked by: xiaohebei
Thank you for your question. We do not currently have any data or product citations using either of these Dnmt3a antibodies in pig samples. Please contact our Technical Service Department and we can assist you with predicting cross reactivity with pig based on sequence analysis of the antibody’s antigens.
Answered by: Technical Support
Date published: 2019-09-11

Have you tested if anti-Dnmt3a (A-10) detects mouse Dnmt3A (endogenous) by WB or IP? You have anti-Dnmt3a (C-12) for mouse Dnmt3a, but I have concern w the exclusive cytoplasmic staining (no nuclei stain) w C-12 antibody shown in your datasheet.

Asked by: hy150
Thank you for your question. We recently published Western blot data in mouse cell lines, NIH/3T3, RAW 264.7 and 3T3-L1 using this mouse monoclonal antibody, Dnmt3a (A-10): sc-373905. We will be updating our species reactivity to include mouse for this antibody.
Answered by: Technical Support
Date published: 2017-11-02

If using the Li-Cor system, what is the recommended secondary for this antibody?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by from Great Antibody for WB Dnmt3a (A-10) gives a beautiful band in positive control HeLa Whole Cell Lysate: sc-2200.
Date published: 2017-01-11
Rated 5 out of 5 by from Good Good, clear band in WB in MIA PaCa-2 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Published data for IP Published data for IP, WB, and CoIP in human Daudi cells and HEK293 cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Diffuse band in WB in human Dnmt3a transfected Diffuse band in WB in human Dnmt3a transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-03-09
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