Dnmt2 Antibody (D-9)recommended for detection of Dnmt2 of mouse, rat and human origin by WB, IP, IF and ELISA

Dnmt2 Antibody (D-9): sc-365001

Dnmt2 Antibody (D-9) is rated 3.8 out of 5 by 5.
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Datasheets

    • Dnmt2 Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 121-391 of Dnmt2 of human origin
    • Dnmt2 Antibody (D-9) is recommended for detection of Dnmt2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Dnmt2 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dnmt2 (D-9): sc-365001.
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What is the recommended starting dilution for Western blot for sc-365001: Dnmt2 (D-9) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

Is the antibody working in IHC(P)

Asked by: KDW0702
Thank you for your question. We have not yet tested Dnmt2 Antibody (D-9): sc-365001 for IHC(P), so we do not know if it is suitable for this application or not. Based on Western blot positive data, it is currently warrantied for IF, or IF or IHC on fresh or frozen tissue sections or cells fixed in acetone or methanol.
Answered by: Technical Support
Date published: 2016-12-20
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Rated 1 out of 5 by from Not for human B cells This antibody recognizes a crossreactive bands in 293T and B-lymphocyte whole cell extract at ~35 kDa. Furthermore, a major crossreactive band at ~70 kDa is detected in human B cells. This antibody does recognize DNMT2, but much weaker.
Date published: 2018-10-23
Rated 4 out of 5 by from a good antibody for WB This antibody works for WB (1:500 dilution, 40 ug HeLa lysate). It does not appear to work for IF (1:200 dilution, formaldehyde-fixed HeLa)
Date published: 2017-07-21
Rated 5 out of 5 by from nice atibody it is a very nice antibody, best results i have got!
Date published: 2017-07-11
Rated 4 out of 5 by from Thick band obtained in WB when used with Thick band obtained in WB when used with human Dnmt2 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Published data in human THP Published data in human THP-1 monocytes using this antibody for Western blot analysis -SCBT Publication Review
Date published: 2014-02-25
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