Dnmt1 Antibody (H-12)recommended for detection of Dnmt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Dnmt1 Antibody (H-12): sc-271729

Dnmt1 Antibody (H-12) is rated 4.4 out of 5 by 21.
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Datasheets

    • Dnmt1 Antibody (H-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1317-1616 mapping near the C-terminus of Dnmt1 of human origin
    • Dnmt1 Antibody (H-12) is recommended for detection of Dnmt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Dnmt1 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dnmt1 (H-12): sc-271729.
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I would like to test antibody for DNA methyltransferase. I am going to buy Dnmt3b (G-9): sc-376043 from your company. Could I get a free 10 µg sample of Dnmt1 (H-12): sc-271729 & Dnmt3a (C-12): sc-365769 ? Thank you so much!

Asked by: snuha
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-21

I need an antibody to perform ChIP assay but since the DNMT1 antibody H-300 has been discontinued, I was wondering if the H-12 can also be used for ChIP although this application is not mentioned in the description

Asked by: JeanneD
Thank you for your question. Unfortunately, this mouse monoclonal, Dnmt1 Antibody (H-12): sc-271729, has not yet been confirmed to work in ChIP applications. For a limited time, we are offering a free 10 µg sample of the antibody. Please contact our Technical Service Department if you would like to request a free sample to test in your intended application. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 3 out of 5 by from funziona benino si vede un segnale ma solo con elevata esposizione
Date published: 2019-02-23
Rated 3 out of 5 by from Not suitable for rat protein 1:100 diluted for wb of rat heart protein ,the band is weak
Date published: 2018-03-19
Rated 4 out of 5 by from Good for wb 1:100 diluted for wb of rat protein ,the band is clear,but a little weak
Date published: 2018-02-13
Rated 5 out of 5 by from We Got the Specific Staining We bought Dnmt1 antibody a few months ago and tested human and mouse cells, they were very satisfied with the performance of the antibodies.
Date published: 2017-10-28
Rated 4 out of 5 by from not very good It's not good to do western blot, I can not get band.
Date published: 2017-08-19
Rated 5 out of 5 by from a relatively good antibody for WB This antibody generates a good signal at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate).
Date published: 2017-07-30
Rated 5 out of 5 by from Very good antibody for WB Gives a sharp band at the expected size. Used in a blot with 100K HCT116 cells. Used 1:1,000 in 5% milk.
Date published: 2017-07-12
Rated 5 out of 5 by from nice antibody Antibody has good quality in Western blot, it works well.
Date published: 2017-07-11
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