Anti-Dlx-2 Antibody (WQ6) recommended for detection of Dlx-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Dlx-2 Antibody (WQ6): sc-81960

Anti-Dlx-2 Antibody (WQ6) is rated 3.0 out of 5 by 2.
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Datasheets

    • Dlx-2 Antibody (WQ6) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant Dlx-2 of human origin
    • recommended for detection of Dlx-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Dlx-2 Antibody (WQ6). This work is in progress.
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Has this antibody been tested on fetal brain paraffin sections (human or mouse)?

Asked by: Ayman
Thank you very much for the question! We have not yet tested fetal brain sections with this antibody clone and we currently do not list any corresponding product citation. Our labs performed immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue and the antibody was showing a nuclear localization. We suggest using a starting dilution of 1:50 for this technique. If you have any further questions, please contact our Technical Service team. You can find the corresponding contact information, by clicking the following link: https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support Europe
Date published: 2017-09-18

Can sc-81960: Dlx-2 (WQ6) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, sc-81960: Dlx-2 (WQ6) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Produced good immunoperoxidase nuclear staining Produced good immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-01-11
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