Dihydrofluorescein diacetate is a chemical used for intracellular staining. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2',7'-dichlorodihydrofluorescein (sc-477276) and Dihydrorhodamine 123 (sc-203027). Dihydrofluorescein diacetate is first hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by hydrogen peroxide (sc-203336).
1. Hempel, S L., et al., 1999. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free radical biology & medicine. 27(1-2): 146-59. PMID: 10443931