DihydroethidiumAn effective dye used to measure oxygen reduction in phagocytes during respiratory burst

Dihydroethidium (CAS 104821-25-2)

Dihydroethidium | CAS 104821-25-2 is rated 5.0 out of 5 by 2.
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Synonym: DHE; Hydroethidine; 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine
Application: An effective dye used to measure oxygen reduction in phagocytes during respiratory burst
CAS Number: 104821-25-2
Purity: ≥95%
Molecular Weight: 315.41
Molecular Formula: C21H21N3
* Refer to Certificate of Analysis for lot specific data (including water content).
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Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen.


References

1. Bucana, C.D., et al. 1990. Exp. Cell Res. 190: 69-75. PMID: 2387329
2. Rothe, G. and Valet, G. 1990. J. Leukoc. Biol. 47: 440-448. PMID: 2159514
3. Perticarari, S., et al. 1991. Cytometry. 12: 687-693. PMID: 1782835
4. Carter, W.O., et al. 1994. J. Leukoc. Biol. 55: 253-258. PMID: 8301222
5. Mancini, M., et al. 1997. J. Cell Biol. 138: 449-469. PMID: 9230085
6. Haugen, T.S., et al. 1999. Eur. Respir. J. 14: 1100-1105. PMID: 10596697

Physical State :
Solid
Solubility :
Soluble in DMSO, DMF, acetonitrile, methanol, and chloroform (20 mg/ml).
Storage :
Store at -20° C
Melting Point :
214.14° C (Predicted)
Boiling Point :
580.4° C at 760 mmHg (Predicted)
Density :
1.20 g/cm3 (Predicted)
Refractive Index :
n20D 1.68 (Predicted)
Fluoresence :
λex 358 nm, λem 461 nm; λex 392 nm, λem 410 nm in methanol
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SF7935000
PubChem CID :
128682
MDL Number :
MFCD00077335
Beilstein Registry :
5107482
SMILES :
CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4

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Dihydroethidium  Product Citations

See how others have used Dihydroethidium. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 12 total

PMID: # 30880248  Liu, T.|Zhang, J.|Han, X.|Xu, J.|Wu, Y.|Fang, J.| et al. 2019. Free Radic. Biol. Med. 135: 216-226.

PMID: # 28474156  Shen, H. et al. 2017. Inflammation. 40: 1319-1330.

PMID: # 27777067  Zhang, J. et al. 2017. Biochim. Biophys. Acta. 1863: 129-138.

PMID: # 26686580  Zhang, J. et al. 2016. Biochem. Pharmacol. 102: 34-44.

PMID: # 27713551  Sun, W. et al. 2016. Sci Rep. 6: 34246.

PMID: # 27261193  Wu, B. et al. 2016. Free Radic. Biol. Med. 97: 179-191.

PMID: # 27916903  Wu, CC.|Huang, YF.|Hsieh, CP.|Chueh, PJ.|Chen, YL.| et al. 2016. Molecules. 21:

PMID: # 24445059  Bruder-Nascimento, T. et al. 2014. J. Mol. Cell. Cardiol. 66: 18-26.

PMID: # 24407164  Duan, D. et al. 2014. Free radical biology & medicine. 69: 15-25.

PMID: # 23856291  Lee, HN. et al. 2013. Biochemical pharmacology. 86: 759-69.

Citations 1 to 10 of 12 total

Is there a recommended storage condition once dissolved in DMSO?

Asked by: Io5ay
Protect from light. Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Answered by: Tech Service 4
Date published: 2016-12-17
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Rated 5 out of 5 by from Great Advances I bought it one years ago and am very happpy. Because it readily penetrates plasma membrane to intercept ROS, especially the superoxides.
Date published: 2017-01-04
Rated 5 out of 5 by from Haugen et al Haugen et al. (PubMed ID 10596697) performed flow cytometry with the dye, dihydroethidium, to analyze the production of intracellular oxidants in alveolar macrophages and blood leukocytes. -SCBT Publication Review
Date published: 2015-03-07
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