Anti-delta Tubulin Antibody (A-1) recommended for detection of δ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-δ Tubulin Antibody (A-1): sc-25259

Anti-δ Tubulin Antibody (A-1) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-δ Tubulin Antibody (A-1) is a mouse monoclonal IgG2a κ δ Tubulin antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 154-450 of δ Tubulin of human origin
    • Anti-delta Tubulin Antibody (A-1) is recommended for detection of δ Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-delta Tubulin Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of δ Tubulin (A-1): sc-25259.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for δ Tubulin Antibody (A-1) for WB and IHC(P) applications. These reagents are now offered in bundles with δ Tubulin Antibody (A-1) (see ordering information below).
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Can δ Tubulin (A-1): sc-25259 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, δ Tubulin (A-1): sc-25259 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from IF 染色不理想 我试用了delta-tubulin小样, 做的是小鼠的免疫荧光, 未见信号。
Date published: 2018-01-22
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human parathyriod tissue. -SCBT QC
Date published: 2015-02-19
Rated 5 out of 5 by from Great antibody Great antibody, used for WB in various samples: F9, HeLa, K-562 and NIH/3T3 whole cell lysates and rat skeletal muscle and rat testis tissue extracts. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Works very nicely for IF on NIH/3T3 samples Works very nicely for IF on NIH/3T3 samples. -SCBT QC
Date published: 2014-10-23
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