Anti-Delta-4 Antibody (G-12) recommended for detection of precursor and mature Delta-4 of human origin by WB, IP, IF and ELISA

Anti-Delta-4 Antibody (G-12): sc-365429

Anti-Delta-4 Antibody (G-12) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-Delta-4 Antibody (G-12) is a mouse monoclonal IgG2a κ Delta-4 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 171-240 mapping within an N-terminal extracellular domain of Delta-4 of human origin
    • Delta-4 Antibody (G-12) is recommended for detection of precursor and mature Delta-4 of human origin by WB, IP, IF and ELISA
    • Anti-Delta-4 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Delta-4 (G-12): sc-365429.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Delta-4 Antibody (G-12) for WB applications. These reagents are now offered in bundles with Delta-4 Antibody (G-12) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-365429: Delta-4 (G-12) monoclonal antibody?

Asked by: Trav11
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from work well in western blot at 1:200,ov90 cell I am pleasure to have try to this sample,it really works well.thank you
Date published: 2017-09-11
Rated 4 out of 5 by from Good WB pictures single and strong band with human arteriovenous malformation tissue at 1:200 dilution.
Date published: 2016-12-07
Rated 5 out of 5 by from IF staining It works for IF on mouse lung slides. But some staining in blood cells too.
Date published: 2016-11-09
Rated 5 out of 5 by from Produced nice Western blot data of Delta Produced nice Western blot data of Delta-4 expression in K-562 whole cell lysate. -SCBT QC
Date published: 2014-05-03
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