Decanoyl-p-nitroanilide is a synthetic fatty acid amide utilized as a probe for activity of FAAH (fatty acid amide hydrolase), the enzyme primarily responsible for degradation of the endocannabinoid Anandamide (sc-200790). FAAH accepts Decanoyl-p-nitroanilide as a substrate and upon amide hydrolysis releases an equivalent of p-nitroaniline which can be assayed at 382 nm (ε = 13500 M-1cm-1) with a UV-Vis spectrophotometer.
1. Patricelli, M.P., et al. 2001. Biochemistry. 40: 6107-6115. PMID: 11352748
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