DDX3 Antibody (C-4): sc-365768

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Datasheets

- DDX3 Antibody (C-4) is a mouse monoclonal IgG_2b κ DDX3 antibody, cited in 21 publications, provided at 200 µg/ml
- raised against amino acids 1-114 mapping at the N-terminus of DDX3x of human origin
- DDX3 Antibody (C-4) is recommended for detection of DDX3X and DDX3Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DDX3 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DDX3 (C-4): sc-365768.
- m-IgG Fc BP-HRP, m-IgG_2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX3 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with DDX3 Antibody (C-4) (see ordering information below).

SEE ALSO...

Additional DDX Antibodies including DDX1, DDX11, DDX15, DDX16, DDX17, DDX21, DDX25, DDX27, DDX29 and DDX30
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

DDX3 Antibody (C-4) - Western Blotting - Image 359695

DDX3 Antibody (C-4) - Immunohistochemistry - Image 370268

DDX3 Antibody (C-4) - Immunofluorescence - Image 146012

DDX3 Antibody (C-4) - Western Blotting - Image 389745

DDX3 Antibody (C-4) - Western Blotting - Image 389746

DDX3 Antibody (C-4) - Western Blotting - Image 354654

DDX3 Antibody (C-4) - Immunohistochemistry - Image 370269

DDX3 Antibody (C-4) - Western Blotting - Image 295928

DDX3 Antibody (C-4) - Immunohistochemistry - Image 370270

DDX3 Antibody (C-4) - Western Blotting - Image 359696

DDX3 Antibody (C-4) - Western Blotting - Image 144815

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DDX3 Antibody (C-4) is an IgG_2b κ mouse monoclonal DDX3 antibody (also designated DDX3X antibody) suitable for the detection of the DDX3 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. DDX3 Antibody (C-4) is available as both the non-conjugated anti-DDX3 antibody form, as well as multiple conjugated forms of anti-DDX3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 (DEAD box polypeptide 3) is involved in RNA metabolism. Two DDX3 paralogs are found in humans; DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	DDX3X	Entrez Gene ID: 1654	Xp11.4	XM_011543892, XM_017029313, XM_017029314, NM_001193416, NM_001193417, NM_001356, NM_024005	UniProt ID: O00571	300160
Human	DDX3Y	Entrez Gene ID: 8653	Yq11.21	NM_001122665, NM_001302552, NM_001324195, NM_004660	UniProt ID: O15523	400010
Mouse	Ddx3x	Entrez Gene ID: 13205	X A1.1	XM_006527566, NM_010028	UniProt ID: Q62167	N/A
Mouse	Ddx3y	Entrez Gene ID: 26900	Y A1	XM_006531603, XM_006531607, XM_011248592, NM_012008	UniProt ID: Q62095	N/A

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Protocols Online

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX3 Antibody (C-4) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX3 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX3 Antibody (C-4) Specifications

Alternate Product Name

DDX3

Product Clone Name

C-4

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

1-114

Epitope Target

DDX3x

Epitope Species

human

Product Reactivity/Detection

DDX3X and DDX3Y

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

21 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG_2b Binding Proteins

m-IgG_2b BP-HRP: sc-542741 (Mouse IgG_2b binding protein conjugated to horseradish peroxidase)
m-IgG_2b BP-B: sc-542744 (Mouse IgG_2b binding protein conjugated to biotin)
m-IgG_2b BP-FITC: sc-542743 (Mouse IgG_2b binding protein conjugated to fluorescein isothiocyanate)
m-IgG_2b BP-CFL 488: sc-542745 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 488)
m-IgG_2b BP-CFL 555: sc-542746 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 555)
m-IgG_2b BP-CFL 594: sc-542747 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 594)
m-IgG_2b BP-CFL 647: sc-542748 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 647)
m-IgG_2b BP-CFL 680: sc-542749 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 680)
m-IgG_2b BP-CFL 790: sc-542750 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 790)

Mouse IgG Kappa Light Chain Binding Proteins

m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

Drosophila

Xenopus

Arabidopsis

C. elegans

Clostridium

Campylobacter

Heliobacter pylori

Hepatitis

Klebsiella

M. tuberculosis

Plasmodium

Pseudomonas

Saccharomyces cerevisiae

Salmonella

Schistosoma

Shigella

Staphylococcus

The preferred DDX3 antibody is DDX3 Antibody (2253C5a): sc-81247.

What is the total volume provided of DDX3 (C-4): sc-365768 monoclonal antibody?

Asked by: jenni

Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Answered by: Technical Support 15

Date published: 2017-01-25

Rated 5 out of 5 by cjMara from Excellent antibody The C-4 clone is an excellent choice for Western blot yielding specific and consistent results.

Date published: 2017-01-13

Rated 5 out of 5 by Bin L from High specificity I bought this several weeks ago, and this antibody fit my demand well, great product!

Date published: 2016-10-27

Rated 5 out of 5 by JM from Good specificity in WB in NIH/3T3 Good specificity in WB in NIH/3T3, A-431, Jurkat and PC-12 nuclear extracts. -SCBT QC

Date published: 2015-06-30

Rated 5 out of 5 by SH from Produced superb immunofluorescence nucleolar Produced superb immunofluorescence nucleolar and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC

Date published: 2015-05-21

Rated 5 out of 5 by MC from Robust signal in WB in HeLa whole cell lysate Robust signal in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2015-02-22

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