DDX3 Antibody (2253C5a): sc-81247

5.0(6)

- DDX3 Antibody (2253C5a) is a mouse monoclonal IgG₁, cited in 10 publications, provided at 100 µg/ml
- raised against a recombinant protein corresponding to the N-terminal region of DDX3 of human origin
- recommended for detection of DDX3X of mouse, rat and human origin by WB and IP
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DDX3 (2253C5a): sc-81247.
- m-IgG Fc BP-HRP, m-IgG₁ BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX3 Antibody (2253C5a) for WB applications. These reagents are now offered in bundles with DDX3 Antibody (2253C5a) (see ordering information below).

References:

DDX3, a DEAD box RNA helicase with tumor growth-suppressive property and transcriptional regulation activity of the p21waf1/cip1 promoter, is a candidate tumor suppressor. | Chao, CH., et al. 2006. Cancer Res. 66: 6579-88. PMID: 16818630
Human DEAD-box ATPase DDX3 shows a relaxed nucleoside substrate specificity. | Franca, R., et al. 2007. Proteins. 67: 1128-37. PMID: 17357160
Phosphorylation of threonine 204 of DEAD-box RNA helicase DDX3 by cyclin B/cdc2 in vitro. | Sekiguchi, T., et al. 2007. Biochem Biophys Res Commun. 356: 668-73. PMID: 17379183
Expression, purification, crystallization and preliminary X-ray diffraction analysis of the DDX3 RNA helicase domain. | Rodamilans, B. and Montoya, G. 2007. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 283-6. PMID: 17401195
Crystal structure of conserved domains 1 and 2 of the human DEAD-box helicase DDX3X in complex with the mononucleotide AMP. | Högbom, M., et al. 2007. J Mol Biol. 372: 150-9. PMID: 17631897
Transcriptional and post-transcriptional regulation of HIV-1 gene expression: role of cellular factors for Tat and Rev. | Nekhai, S. and Jeang, KT. 2006. Future Microbiol. 1: 417-26. PMID: 17661632
Candidate tumor suppressor DDX3 RNA helicase specifically represses cap-dependent translation by acting as an eIF4E inhibitory protein. | Shih, JW., et al. 2008. Oncogene. 27: 700-14. PMID: 17667941
DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication. | Ariumi, Y., et al. 2007. J Virol. 81: 13922-6. PMID: 17855521
The DDX3 subfamily of the DEAD box helicases: divergent roles as unveiled by studying different organisms and in vitro assays. | Rosner, A. and Rinkevich, B. 2007. Curr Med Chem. 14: 2517-25. PMID: 17979704

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	DDX3X	Entrez Gene ID: 1654	Xp11.4	XM_011543892, XM_017029313, XM_017029314, NM_001193416, NM_001193417, NM_001356, NM_024005	UniProt ID: O00571	300160
Mouse	Ddx3x	Entrez Gene ID: 13205	X A1.1	XM_006527566, NM_010028	UniProt ID: Q62167	N/A

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX3 Antibody (2253C5a) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX3 Antibody (2253C5a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX3 Antibody (2253C5a) Specifications

Alternate Product Name

DDX3

Product Clone Name

2253C5a

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

DDX3

Epitope Species

human

Product Reactivity/Detection

DDX3X

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP

Vialing Information

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations

10 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG₁ Binding Proteins

m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Mouse IgG Kappa Light Chain Binding Proteins

m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-DDX3 Antibody (2253C5a) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero

Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.

Answered by: Technical Support 9

Date published: 2017-02-16

Rated 5 out of 5 by AbPolly from No background Strong and clean results seen in Western Blot - no additional bands or background staining.

Date published: 2017-01-27

Rated 5 out of 5 by HC from Good specificity in WB in F2408 whole cell lysate Good specificity in WB in F2408 whole cell lysate. -SCBT QC

Date published: 2015-06-25

Rated 5 out of 5 by HF from Robust signal in WB in Jurkat and PC Robust signal in WB in Jurkat and PC-12 whole cell lysates and K-562 nuclear extract. -SCBT QC

Date published: 2015-03-11

Rated 5 out of 5 by SH from Positive signal in WB in NIH/3T3 whole cell lysate Positive signal in WB in NIH/3T3 whole cell lysate. -SCBT QC

Date published: 2015-02-15

Rated 5 out of 5 by SS from Worked well in WB in human recombinant ZnT Worked well in WB in human recombinant ZnT-9 fusion protein. -SCBT QC

Date published: 2015-01-05

Rated 5 out of 5 by SF from Published IHCP data with human Oral squamous Published IHCP data with human Oral squamous cell carcinoma (OSCC) - SCBT Publication Review

Date published: 2014-03-24

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