DDX17 Antibody (H-7): sc-398168

4.7(7)

- DDX17 Antibody (H-7) is a mouse monoclonal IgG_2a κ DDX17 antibody, cited in 10 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 143-155 within an internal region of DDX17 of human origin
- DDX17 Antibody (H-7) is recommended for detection of DDX17 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DDX17 Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DDX17 (H-7): sc-398168.
- m-IgG Fc BP-HRP, m-IgG_2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DDX17 Antibody (H-7) for WB and IHC(P) applications. These reagents are now offered in bundles with DDX17 Antibody (H-7) (see ordering information below).

References:

The mRNA of DEAD box protein p72 is alternatively translated into an 82-kDa RNA helicase. | Uhlmann-Schiffler, H., et al. 2002. J Biol Chem. 277: 1066-75. PMID: 11675387
Reduced levels of DEAD-box proteins DBP-RB and p72 in fetal Down syndrome brains. | Kircher, SG., et al. 2002. Neurochem Res. 27: 1141-6. PMID: 12462412
The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner. | Wilson, BJ., et al. 2004. BMC Mol Biol. 5: 11. PMID: 15298701
RNA helicases: regulators of differentiation. | Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
Redundant role of DEAD box proteins p68 (Ddx5) and p72/p82 (Ddx17) in ribosome biogenesis and cell proliferation. | Jalal, C., et al. 2007. Nucleic Acids Res. 35: 3590-601. PMID: 17485482
Involvement of RNA helicases p68 and p72 in colon cancer. | Shin, S., et al. 2007. Cancer Res. 67: 7572-8. PMID: 17699760
The DEAD box RNA helicases p68 (Ddx5) and p72 (Ddx17): novel transcriptional co-regulators. | Fuller-Pace, FV. and Ali, S. 2008. Biochem Soc Trans. 36: 609-12. PMID: 18631126
p72: a human nuclear DEAD box protein highly related to p68. | Lamm, GM., et al. 1996. Nucleic Acids Res. 24: 3739-47. PMID: 8871553

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	DDX17	Entrez Gene ID: 10521	22q13.1	NM_001098504, NM_006386, NM_001098505, NM_030881	UniProt ID: Q92841	608469
Mouse	Ddx17	Entrez Gene ID: 67040	15 E1	XM_006521290, NM_001040187, NM_152806, NM_199079, NM_199080	UniProt ID: Q501J6	N/A

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX17 Antibody (H-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX17 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX17 Antibody (H-7) Specifications

Alternate Product Name

DDX17

Product Clone Name

H-7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

143-155

Epitope Target

DDX17

Epitope Species

human

Product Reactivity/Detection

DDX17

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

10 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG_2a Binding Proteins

m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Mouse IgG Kappa Light Chain Binding Proteins

m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase P: Blocking Peptide PE: Phycoerythrin

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-DDX17 Antibody (H-7) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

What application is the blocking peptide sc-398168 P appropriate for?

Asked by: cjMara

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by xuehui from 条带很清晰申请了试用装，然后结果不错。条带非常清晰，再我们的细胞中表达的也非常的丰富。要买商品装啦，希望可以得到优惠券

Date published: 2020-10-12

Rated 5 out of 5 by Boost from Great Veru good signal in WB of total lysates of human cell lines.

Date published: 2020-05-26

Rated 4 out of 5 by Blotter from Very strong bands, nice Antibody Very strong bands even with a 1:7500 dilution over night.

Date published: 2017-11-28

Rated 5 out of 5 by Lee L from Western blot The antibody is really good! The western blot band is very clear!

Date published: 2017-09-18

Rated 4 out of 5 by JL from Blowout band observed by WB using NIH/3T3 Blowout band observed by WB using NIH/3T3 whole cell lysate. -SCBT QC

Date published: 2015-06-08

Rated 5 out of 5 by SH from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC

Date published: 2015-04-23

Rated 5 out of 5 by QZ from Positive detection of endogenously expressed Positive detection of endogenously expressed DDX17 protein in Jurkat, LADMAC, HeLa, MCF7, K-562 and A-431 cell lines through analysis by Western Blot. -SCBT QC

Date published: 2015-02-04

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DDX17 Antibody (H-7): sc-398168

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References:

DDX17 Antibody (H-7) Specifications

Alternate Product Name

Product Clone Name

Classification

Clonality

Antibody Isotype

Amino Acids

Epitope Target

Epitope Species

Product Reactivity/Detection

Species Reactivity/Detection

Additional Mammalian Reactivity

Additional Non-Mammalian Reactivity

Applications

Vialing Information

Research Citations

Recommended Secondary Detection Reagents

Available Conjugates/Forms

What application is the blocking peptide sc-398168 P appropriate for?